%A Xuan JIA, Hai YAN, Zijing WANG, Huanju HE, Qianqian XU, Haiou WANG, Chunhua YIN, Liqin LIU
%T Carbon dioxide fixation by Chlorella sp. USTB-01 with a fermentor-helical combined photobioreactor
%0 Journal Article
%D 2011
%J Front. Environ. Sci. Eng.
%J Frontiers of Environmental Science & Engineering
%@ 2095-2201
%R 10.1007/s11783-010-0223-0
%P 402-408
%V 5
%N 3
%U {https://journal.hep.com.cn/fese/EN/10.1007/s11783-010-0223-0
%8 2011-09-05
%X
A promising microalgal strain isolated from fresh water, which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting, was identified as Chlorella sp. USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences. In the heterotrophic batch culture, more than 20.0 g·L-1 of cell dry weight concentration (DWC) of Chlorella sp. USTB-01 was obtained at day 5, and which was used directly to seed the autotrophic culture. A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp. USTB-01 for the fixation of carbon dioxide (CO2). It showed that the autotrophic growth of Chlorella sp. USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g·L-1 was obtained at day 6. The highest CO2 fixation of 95% appeared on day 1 in the exponential growth phases of Chlorella sp. USTB-01 and 49.8% protein was found in the harvested microalgal cells.