%A Xuan JIA, Hai YAN, Zijing WANG, Huanju HE, Qianqian XU, Haiou WANG, Chunhua YIN, Liqin LIU %T Carbon dioxide fixation by Chlorella sp. USTB-01 with a fermentor-helical combined photobioreactor %0 Journal Article %D 2011 %J Front. Environ. Sci. Eng. %J Frontiers of Environmental Science & Engineering %@ 2095-2201 %R 10.1007/s11783-010-0223-0 %P 402-408 %V 5 %N 3 %U {https://journal.hep.com.cn/fese/EN/10.1007/s11783-010-0223-0 %8 2011-09-05 %X

A promising microalgal strain isolated from fresh water, which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting, was identified as Chlorella sp. USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences. In the heterotrophic batch culture, more than 20.0 g·L-1 of cell dry weight concentration (DWC) of Chlorella sp. USTB-01 was obtained at day 5, and which was used directly to seed the autotrophic culture. A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp. USTB-01 for the fixation of carbon dioxide (CO2). It showed that the autotrophic growth of Chlorella sp. USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g·L-1 was obtained at day 6. The highest CO2 fixation of 95% appeared on day 1 in the exponential growth phases of Chlorella sp. USTB-01 and 49.8% protein was found in the harvested microalgal cells.