Arsenic release by indigenous bacteria Bacillus cereus from aquifer sediments at Datong Basin, northern China

Abstract

Endemic arsenic poisoning due to long-term drinking of high arsenic groundwater has been reported in Datong Basin, northern China. To investigate the effects of microbial activities on arsenic mobilization in contaminated aquifers, Bacillus cereus (B. cereus) isolated from high arsenic aquifer sediments of the basin was used in our microcosm experiments. The arsenic concentration in the treatment with both bacteria and sodium citrate or glucose had a rapid increase in the first 18 d, and then, it declined. Supplemented with bacteria only, the concentration could increase on the second day. By contrast, the arsenic concentration in the treatment supplemented with sodium citrate or glucose was kept very low. These results indicate that bacterial activities promoted the release of arsenic in the sediments. Bacterial activities also influenced other geochemical parameters of the aqueous phase, such as pH, Eh, and the concentrations of dissolved Fe, Mn, and Al that are important controls on arsenic release. The removal of Fe, Mn, and Al from sediment samples was observed with the presence of B. cereus. The effects of microbial activities on Fe, Mn, and Al release were nearly the same as those on As mobilization. The pH values of the treatments inoculated with bacteria were lower than those without bacteria, still at alkaline levels. With the decrease of Eh values in treatments inoculated with bacteria, the microcosms became more reducing and are thus favorable for arsenic release.

Keywords

arsenic / groundwater / indigenous bacteria / redox / biogeochemistry

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Acknowledgements

The research work was financially supported by the Special Fund for Basic Scientific Research of Central Colleges, China University of Geosciences (Wuhan) (CUGL090220), MOE Key Laboratory of Biogeology and Environmental Geology (BGEGF 200817), the National Natural Science Foundation of China (Grant Nos. 40425001, 40830748, and 40802058), Ministry of Science and Technology of China (2008DFA20950), and the 111 project (B08030).

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