Frontiers of Chemical Science and Engineering >

2009 , Vol. 3 >Issue 4: 375 - 382

DOI: https://doi.org/10.1007/s11705-009-0247-9

Research articles

Application of high-speed counter-current chromatography coupled with high performance liquid chromatography for the separation and purification of Quercetin-3- O -sambubioside from the leaves of Nelumbo nucifera

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  • State Key Lab of Food Science and Technology, Institute for Advanced Study, Nanchang University, Nanchang 330047, China;

Published date: 05 Dec 2009

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Abstract

Quercetin-3-O-sambubioside [Quercetin-3-O-β-D-xylopyranosyl (1→2)-β-D-glucopyranoside] was separated and purified by semi-preparative high-speed counter-current chromatography (HSCCC) with a two-phase-solvent system composed of ethyl acetate-n-butanol–water (4∶1∶5, v/v) from the leaves of Nelumbo nucifera (Lotus). A total of 5.0mg of the targeted compound with a purity of 98.6% as determined by high performance liquid chromatography (HPLC) was obtained from 100m g of the crude extract cleaned up by AB-8 macroporous resin in a one-step separation. Quercetin-3-O-sambubioside was a novel flavonoid glycoside from the leaves of Nelumbo nucifera, and its chemical structure was identified by means of ESI-MS, 1D NMR and 2D NMR.

Cite this article

Shengguo DENG, Zeyuan DENG, Yawei FAN, Jing LI, Rong LIU, Dongmei XIONG, . Application of high-speed counter-current chromatography coupled with high performance liquid chromatography for the separation and purification of Quercetin-3- O -sambubioside from the leaves of Nelumbo nucifera[J]. Frontiers of Chemical Science and Engineering, 2009 , 3(4) : 375 -382 . DOI: 10.1007/s11705-009-0247-9

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