Improved energy recovery from dark fermented cane molasses using microbial fuel cells

Abstract

A major limitation associated with fermentative hydrogen production is the low substrate conversion efficiency. This limitation can be overcome by integrating the process with a microbial fuel cell (MFC) which converts the residual energy of the substrate to electricity. Studies were carried out to check the feasibility of this integration. Biohydrogen was produced from the fermentation of cane molasses in both batch and continuous modes. A maximum yield of about 8.23 mol H₂/kg COD_removed was observed in the batch process compared to 11.6 mol H₂/kg COD_removed in the continuous process. The spent fermentation media was then used as a substrate in an MFC for electricity generation. The MFC parameters such as the initial anolyte pH, the substrate concentration and the effect of pre-treatment were studied and optimized to maximize coulombic efficiency. Reductions in COD and total carbohydrates were about 85% and 88% respectively. A power output of 3.02 W/m³ was obtained with an anolyte pH of 7.5 using alkali pre-treated spent media. The results show that integrating a MFC with dark fermentation is a promising way to utilize the substrate energy.

Key words： dark fermentation; biohydrogen; microbial fuel cell; volatile fatty acid; anolyte

Acknowledgements

The financial support received from the Council of Scientific & Industrial Research (CSIR) and the Ministry of New and Renewable Energy (MNRE) of India is duly acknowledged.

References

1 Elam C C, Padró C E G, Sandrock G, Luzzi A, Lindblad P, Hagen E F. Realizing the hydrogen future: the International Energy Agency’s efforts to advance hydrogen energy technologies. International Journal of Hydrogen Energy, 2003, 28(6): 601–607

2 Nayak B K, Pandit S, Das D. Biohydrogen. In: Kennes C, Veiga ría C, editors. Air Pollution Prevention and Control. John Wiley & Sons Ltd, 2013, 345–381

3 Oh Y K, Raj S M, Jung G Y, Park S. Current status of the metabolic engineering of microorganisms for biohydrogen production. Bioresource Technology, 2011, 102(18): 8357–8367

4 Das D, Veziroǧlu T N. Hydrogen production by biological processes: A survey of literature. International Journal of Hydrogen Energy, 2001, 26(1): 13–28

5 Levin D B, Pitt L, Love M. Biohydrogen production: Prospects and limitations to practical application. International Journal of Hydrogen Energy, 2004, 29(2): 173–185

6 Jung G Y, Jung H O, Kim J R, Ahn Y, Park S. Isolation and characterization of Rhodopseudomonas palustris P4 which utilizes CO with the production of H₂. Biotechnology Letters, 1999, 21(6): 525–529

7 Benemann J. Hydrogen biotechnology: progress and prospects. Nature Biotechnology, 1996, 14(9): 1101–1103

8 Mohan S V, Srikanth S, Velvizhi G, Babu M L. Microbial Fuel Cells for Sustainable Bioenergy Generation: Principles and Perspective Applications. In: Gupta V K, Tuohy M G, eds. Biofuel Technologies. Berlin: Springer Berlin Heidelberg, 2013, 335–368

9 Momirlan M, Veziroglu T. Current status of hydrogen energy. Renewable & Sustainable Energy Reviews, 2002, 6(1–2): 141–179

10 Lovley D R. The microbe electric: conversion of organic matter to electricity. Current Opinion in Biotechnology, 2008, 19(6): 564–571

11 Logan B E, Regan J M. Electricity-producing bacterial communities in microbial fuel cells. Trends in Microbiology, 2006, 14(12): 512–518

12 Torres C I, Marcus A K, Lee H S, Parameswaran P, Krajmalnik-Brown R, Rittmann B E. A kinetic perspective on extracellular electron transfer by anode-respiring bacteria. FEMS Microbiology Reviews, 2010, 34(1): 3–17

13 Oh S T, Kim J R, Premier G C, Lee T H, Kim C, Sloan W T. Sustainable wastewater treatment: How might microbial fuel cells contribute. Biotechnology Advances, 2010, 28(6): 871–881

14 Guwy A J, Dinsdale R M, Kim J R, Massanet-Nicolau J, Premier G. Fermentative biohydrogen production systems integration. Bioresource Technology, 2011, 102(18): 8534–8542

15 Sharma Y, Li B. Optimizing energy harvest in wastewater treatment by combining anaerobic hydrogen producing biofermentor (HPB) and microbial fuel cell (MFC). International Journal of Hydrogen Energy, 2010, 35(8): 3789–3797

16 Mohanakrishna G, Venkata Mohan S, Sarma P N. Utilizing acid-rich effluents of the fermentative hydrogen production process as a substrate for harnessing bioelectricity: An integrative approach. International Journal of Hydrogen Energy, 2010, 35(8): 3440–3449

17 Wang A, Sun D, Cao G, Wang H, Ren N, Wu W M, Logan B E. Integrated hydrogen production process from cellulose by combining dark fermentation, microbial fuel cells, and a microbial electrolysis cell. Bioresource Technology, 2011, 102(5): 4137–4143

18 Park M J, Jo J H, Park D, Lee D S, Park J M. Comprehensive study on a two-stage anaerobic digestion process for the sequential production of hydrogen and methane from cost-effective molasses. International Journal of Hydrogen Energy, 2010, 35(12): 6194–6202

19 Vatsala T M. Hydrogen production from (cane-molasses) stillage by Citrobacter freundii and its use in improving methanogenesis. International Journal of Hydrogen Energy, 1992, 17(12): 923–927

20 González T, Terrón M C, Yagüe S, Zapico E, Galletti G C, González A E. Pyrolysis/gas chromatography/mass spectrometry monitoring of fungal-biotreated distillery wastewater using Trametes sp. I-62 (CECT 20197). Rapid Communications in Mass Spectrometry, 2000, 14(15): 1417–1424

21 Singhania R R, Patel A K, Christophe G, Fontanille P, Larroche C. Biological upgrading of volatile fatty acids, key intermediates for the valorization of biowaste through dark anaerobic fermentation. Bioresource Technology, 2013, 145: 166–174

22 Poggi-Varaldo H M, Carmona-Martínez A, Vázquez-Larios A L, Solorza-Feria O.Effect of inoculum type on the performance of a microbial fuel cell fed with spent organic extracts from hydrogenogenic fermentation of organic solid wastes. Journal of New Materials for Electrochemical Systems, 2009, 12: 049–054

23 Kumar N, Das D. Enhancement of hydrogen production by Enterobacter cloacae IIT-BT 08. Process Biochemistry, 2000, 35(6): 589–593

24 Kumar N, Das D. Continuous hydrogen production by immobilized Enterobacter cloacae IIT-BT 08 using lignocellulosic materials as solid matrices. Enzyme and Microbial Technology, 2001, 29(4–5): 280–287

25 Khilari S, Pandit S, Ghangrekar M M, Das D, Pradhan D. Graphene supported α-MnO2 nanotubes as a cathode catalyst for improved power generation and wastewater treatment in single-chambered microbial fuel cells. RSC Advances, 2013, 3(21): 7902–7911

26 Behera M, Ghangrekar M M. Performance of microbial fuel cell in response to change in sludge loading rate at different anodic feed pH. Bioresource Technology, 2009, 100(21): 5114–5121

27 Lay J J, Li Y Y, Noike T. Influences of pH and moisture content on the methane production in high-solids sludge digestion. Water Research, 1997, 31(6): 1518–1524

28 Standard Methods for the Examination of Water and Wastewater. 20th Ed. American Public Health Association (APHA), American Water Works Association, Water Pollution Control Federation, Washington DC. 1998, 141

29 Logan B E, Hamelers B, Rozendal R, Schröder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey K. Microbial fuel cells: Methodology and technology. Environmental Science & Technology, 2006, 40(17): 5181–5192

30 Logan B E. Microbial Fuel Cells. 1st ed. Wiley-Interscience, 2008, 216

31 Loewus F A. Improvement in anthrone method for determination of carbohydrates. Analytical Chemistry, 1952, 24(1): 219–219

32 Das D, Veziroğlu T N. Hydrogen production by biological processes: a survey of literature. International Journal of Hydrogen Energy, 2001, 26(1): 13–28

33 Seol E, Kim S, Raj S M, Park S. Comparison of hydrogen-production capability of four different Enterobacteriaceae strains under growing and non-growing conditions. International Journal of Hydrogen Energy, 2008, 33(19): 5169–5175

34 Bringi V, Dale B E. Enhanced yeast immobilization by nutrient starvation. Biotechnology Letters, 1985, 7(12): 905–908

35 Gavala H N, Skiadas I V, Ahring B K. Biological hydrogen production in suspended and attached growth anaerobic reactor systems. International Journal of Hydrogen Energy, 2006, 31(9): 1164–11

36 Gil G C, Chang I S, Kim B H, Kim M, Jang J K, Park H S, Kim H J. Operational parameters affecting the performance of a mediator-less microbial fuel cell. Biosensors & Bioelectronics, 2003, 18(4): 327–334

37 He Z, Huang Y, Manohar A K, Mansfeld F. Effect of electrolyte pH on the rate of the anodic and cathodic reactions in an air-cathode microbial fuel cell. Bioelectrochemistry (Amsterdam, Netherlands), 2008, 74(1): 78–82

38 Ren Z, Ward T E, Regan J M. Electricity production from cellulose in a microbial fuel cell using a defined binary culture. Environmental Science & Technology, 2007, 41(13): 4781–4786

39 Venkata Mohan S, Veer Raghavulu S, Sarma P N. Biochemical evaluation of bioelectricity production process from anaerobic wastewater treatment in a single chambered microbial fuel cell (MFC) employing glass wool membrane. Biosensors & Bioelectronics, 2008, 23(9): 1326–1332

40 Menicucci J, Beyenal H, Marsili E, Veluchamy, Demir G, Lewandowski Z. Veluchamy, Demir G, Lewandowski Z. Procedure for determining maximum sustainable power generated by microbial fuel cells. Environmental Science & Technology, 2006, 40(3): 1062–1068

41 Yuan Y, Zhao B, Zhou S, Zhong S, Zhuang L. Electrocatalytic activity of anodic biofilm responses to pH changes in microbial fuel cells. Bioresource Technology, 2011, 102(13): 6887–6891

42 Kim B H, Chang I S, Gadd G M. Challenges in microbial fuel cell development and operation. Applied Microbiology and Biotechnology, 2007, 76(3): 485–494

43 Kim B H, Chang I S, Gil G C, Park H S, Kim H J. Novel BOD (biological oxygen demand) sensor using mediator-less microbial fuel cell. Biotechnology Letters, 2003, 25(7): 541–545

44 Di Lorenzo M, Curtis T P, Head I M, Scott K. A single-chamber microbial fuel cell as a biosensor for wastewaters. Water Research, 2009, 43(13): 3145–3154

45 Lefebvre O, Tan Z, Kharkwal S, Ng H Y. Effect of increasing anodic NaCl concentration on microbial fuel cell performance. Bioresource Technology, 2012, 112: 336–340