Interfacing biosynthetic CdS with engineered Rhodopseudomonas palustris for efficient visible light-driven CO₂–CH₄ conversion

Abstract

Engineered photosynthetic bacterium Rhodopseudomonas palustris is excellent at one-step CO₂ biomethanation and can use near-infrared light sources, overcoming the limitations of conventional photosynthetic systems. The current study constructed a biohybrid system that deposited CdS nanoparticles on R. palustris. This biohybrid system broadens the capture of sustainable solar energy, achieving a 155 nmol·mL^–1 biological CH₄ production under full visible light irradiation, 13.4-fold of that by the pure R. palustris. The transcriptome profiles revealed that gene expression related to photosynthetic electron transfer chain, nitrogenase, nanofilaments, and redox stress defense was activated. Accordingly, we attributed the much-enhanced CO₂ biomethanation in the biohybrid system to the remarkable increase in the intracellular reducing power and the stronger rigidity of the cells assisted by photoexcited electrons from CdS nanoparticles. Our discovery offers insight and a promising strategy for improving the current CO₂–CH₄ biomanufacturing system.

Key words： CO₂ methanation; Rhodopseudomonas palustris; CdS nanoparticles; green catalysis

Competing interests

The authors declare that they have no competing interests.

Acknowledgements

This work was supported by the 2022 Carbon Dafeng and Carbon Neutral Science and Technology Innovation Special Fund in Jiangsu Province (Grant No. BK20220003), the National Natural Science Foundation of China (Grant No. 32371538), the Special Funds for Jiangsu Provincial Science and technology plan (Grant No. BZ2022052), the Jiangsu Agriculture Science and Technology Innovation Fund (Grant No. CX(21)2015), and the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (Grant No. XTD2204).

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11705-024-2460-y and is accessible for authorized users.

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