Frontiers of Chemical Science and Engineering >

2011 , Vol. 5 >Issue 2: 238 - 244

DOI: https://doi.org/10.1007/s11705-010-0002-2

RESEARCH ARTICLE

Anti-hyperglycemic effect of the polysaccharide fraction isolated from mactra veneriformis

  • Lingchong WANG 1,2 ,
  • Hao WU , 1,2 ,
  • Nian CHANG 1 ,
  • Kun ZHANG 1
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  • 1. College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing 210046, China
  • 2. Research Center of Marine Drug in Jiangsu Province, Nanjing 210046, China

Received date: 20 Jan 2010

Accepted date: 27 Mar 2010

Published date: 05 Jun 2011

Copyright

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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Abstract

Total macromolecule extract was obtained from the soft body of Mactra veneriformis by the coupling techniques of decoction and alcohol precipitation. The extract was deproteinized with an ion exchange column, and resulted in the purifying of the crude polysaccharide fraction. It was found by chemical analysis that the crude polysaccharide part is composed of abundant polysaccharides (>95%) and few proteins (<1%). Furthermore, only one type of monosaccharide, glucose, was detected from its hydrolytes by thin-layer chromatography, indicating that the polysaccharides might be analogs of glucosan. The anti-hyperglycemia effects of the crude polysaccharide part were preliminarily investigated using several pharmacological methods in normal and diabetic mice. Animal experimental results showed that the crude polysaccharide fraction exhibited proper glycemia inhibition activity, and 300 mg/kg-weight dose has the optimal effect among all the studied doses. It is concluded that the crude polysaccharide fraction can be explored as a novel health product that possesses potential as an anti-hyperglycemic agent.

Key words： anti-hyperglycemia; Mactra veneriformis; polysaccharide; monosaccharide composition; oral glucose tolerance test

Cite this article

Lingchong WANG , Hao WU , Nian CHANG , Kun ZHANG . Anti-hyperglycemic effect of the polysaccharide fraction isolated from mactra veneriformis[J]. Frontiers of Chemical Science and Engineering, 2011 , 5(2) : 238 -244 . DOI: 10.1007/s11705-010-0002-2

Acknowledgments

The financial grant for this work was supported by the National Natural Science Foundation of China (Grant No. 30900293) and the R&D Special Fund for Public Welfare Industry from State Ocean Administration of China (No. 200709005). The authors also wish to thank Luan H E and Jin Y for their contributions and active participation, and Mr. Wan X H and Miss Bian H M for technical assistance.

References

Publishing order | Descend order by publishing year | Descend order by cited within
1
Harris M I, Hadden W C, Knowler W C, Bennett P H. Prevalence of diabetes and impaired glucose tolerance and plasma glucose levels in US population aged 20-74 yrDiabetes, 1987, 36(4): 523–534

DOI

2
Stern M P. Diabetes and cardiovascular disease. The “common soil” hypothesis. Diabetes, 1995, 44(4): 369–374

DOI

3
Pan X R, Yang W Y, Li G W, Liu J, Prevalence of diabetes and its risk factors in China, 1994. Diabetes Care, 1997, 20(11): 1664–1669

DOI

4
Blunt J W, Copp B R, Hu W P, Munro M H G, Northcote P T, Prinsep M R. Marine natural products. Natural Product Reports, 2009, 26(2): 170–244

DOI

5
Chang N, Wu H, Wang L, Yao J, Jing Y. Hypoglycemic effect of Mactra Veneriformis extraction. Journal of Nanjing TCM University, 2009, 25(4): 277–280 (in Chinese)

6
Adlam C, Knights J M, Mugridge A, Lindon J C, Baker P R, Beesley J E, Spacey B, Craig G R, Nagy L K. Purification, characterization and immunological properties of the serotype-specific capsular polysaccharide of Pasteurella haemolytica (serotype A1) organisms. J Gen Microbiol, 1984, 130(9): 2415–2426

7
Walker J M. The bicinchoninic acid (BCA) assay for protein quantitation. Methods Mol Biol, 1994, 32: 5–8

8
Houff W H, Christie D R, Beaumont R H. Analysis of aromatic sulfonation reaction mixtures. Analytical Chemistry, 1957, 29(12): 1866–1868

DOI

9
Slodki M E, Wickerham L J. Extracellular polysaccharides and classification of the genus Lipomyces. J Gen Microbiol, 1966, 42(3): 381–385

10
Chen G C, Johnson B R. Improved colorimetric determination of cell wall chitin in wood decay fungi. Appl Environ Microbiol, 1983, 46(1): 13–16

11
Ajabnoor M A. Effect of aloes on blood glucose levels in normal and alloxan diabetic mice. Journal of Ethnopharmacology, 1990, 28(2): 215–220

DOI

12
Senso A, Franco P, Oliveros L, Minguillón C A. Characterization of doubly substituted polysaccharide derivatives. Carbohydrate Research, 2000, 329(2): 367–376

DOI

13
Turgeona S L, Schmittb C, Sanchezc C. Protein-polysaccharide complexes and coacervates. Curr Opin Colloid In, 2007, 12(4-5): 166–178

DOI

14
Melo M R S, Feitosa J P A, Freitasa A L P, de Paula R C M. Isolation and characterization of soluble sulfated polysaccharide from the red seaweed Gracilaria cornea. Carbohydrate Polymers, 2002, 49(4): 491–498

DOI

15
Bankir L, Bardoux P, Ahloulay M. Vasopressin and diabetes mellitus. Nephron, 2001, 87(1): 8–18

DOI

16
McDonald G W, Fisher G F, Burnham C. Reproducibility of the oral glucose tolerance test. Diabetes, 1965, 14: 473–480

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