Frontiers of Chemical Science and Engineering >
Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption
Received date: 25 Dec 2017
Accepted date: 09 May 2018
Published date: 25 Feb 2019
Copyright
The mixed-mode resins for protein adsorption have been prepared by a novel strategy, copolymer grafting. Specially, the copolymer-grafted resins CG-M-A with two functional groups, 5-amino-benzimidazole (ABI) and methacryloxyethyltrimethyl ammonium chloride (METAC), have been prepared through surface-initiated activator generated by electron transfer for atom transfer radical polymerization of METAC and glycidyl methacrylate (GMA), followed by a ring-open reaction to introduce ABI. The charge and hydrophobicity of CG-M-A resins could be controlled by manipulating the addition of METAC and GMA/ABI. Besides, METAC and ABI provided positive effects together in both protein adsorption and elution: dynamic binding capacity of human Immunoglobulin G (hIgG) onto CG-M-A resin with the highest ligand ratio of METAC to ABI is 46.8 mg·g−1 at pH 9 and the elution recovery of hIgG is 97.0% at pH 5. The separation experiment showed that purity and recovery of monoclonal antibody from cell culture supernatant are 96.0% and 86.5%, respectively, indicating that copolymer-grafted mixed-mode resins could be used for antibody purification.
Shenggang Chen , Tao Liu , Ruiqi Yang , Dongqiang Lin , Shanjing Yao . Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption[J]. Frontiers of Chemical Science and Engineering, 2019 , 13(1) : 70 -79 . DOI: 10.1007/s11705-018-1745-4
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