%A Fang Meijuan, Wang Jianfeng, Huang Yaojian, Zhao Yufen
%T Rapid Screening and Identification of Brefeldin A in Endophytic Fungi Using HPLC-MS/MS
%0 Journal Article
%D 2006
%J Front. Chem. China
%J Frontiers of Chemistry in China
%@ 1673-3495
%R 10.1007/s11458-005-0016-3
%P 15-19
%V 1
%N 1
%U {https://journal.hep.com.cn/fcc/EN/10.1007/s11458-005-0016-3
%8 2006-03-05
%X Brefeldin A (BFA; C16H24O4, MW 280), a naturally occurring macrolide, exhibits diverse biological activities, such as antibiotic, antiviral, cytostatic, antimitotic, and antitumor effects. Owing to the wide activities of BFA, there is a need to develop a method for rapid identification of BFA in crude microbial extracts. In this paper, a method has been established and validated for screening and identification of individual as well as total BFA by high-performance liquid chromatography and liquid chromatography/electrospray mass spectrometry in fungal raw materials.