%A Qing LIU,Dongxin FENG,Xiaowu WANG,Yongchen DU %T Cloning and functional analysis of the genes involved in signal transduction in tomato Cf-4-Avr4 pathosystem %0 Journal Article %D 2007 %J Front. Agric. China %J Frontiers of Agriculture in China %@ 1673-7334 %R 10.1007/s11703-007-0009-8 %P 52-57 %V 1 %N 1 %U {https://journal.hep.com.cn/fag/EN/10.1007/s11703-007-0009-8 %8 %X

Hypersensitive response (HR) is one of the most efficient and common resistance mechanisms in plants. Cloning signaling genes are very important to elucidate the resistance mechanisms. A gene in tomato homologous to several resistance proteins in plant was involved in HR and named as RGL (Resistance Gene Like). RGL protein was used as a bait to screen interacting protein(s) from tomato cDNA library through the yeast two-hybrid system. Two interacting proteins were found, which were called as RGLIP-1 and RGLIP2 (RGL Interacting Protein), respectively. RGLIP-1 is a protein of 291 amino acids with significant homology with thylakoid lumen protein. RGLIP-2 is a protein of 248 amino acids with significant homology with transducin protein. Virus-Induced Gene Silencing (VIGS) of the two genes results in a partial and complete suppression of Avr4induced HR, which indicates that both genes are involved in hypersensitive response.