Frontiers of Agriculture in China >

2010 , Vol. 4 >Issue 2: 188 - 194

DOI: https://doi.org/10.1007/s11703-010-0097-8

Research articles

Synthesis of artificial antigens of diethylstilbestrol and preparation of its antibody

Expand
  • College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China;

Published date: 05 Jun 2010

Fold

Abstract

Diethylstilbestrol-hemi-succinate (DES-HS) and diethylstilbestrol-monocarboxylic-propyl-ether (DES-MCPE) were synthesized in different ways. The two kinds of haptens were conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the immuno-antigens and the coating-antigen, respectively. The antibody with high titer was obtained after immunising rabbits. The results showed that the antibody had a high affinity to diethylstilbestrol tested by enzyme-linked immunosorbent assay (ELISA) and Western blot. This study lays a basis for further research on the immune test kit for the DES remnant.

Key words： diethylstilbestrol; hapten; artificial antigen; antibody

Cite this article

Qiujia FAN, Xianghong WANG, Qunbiao ZHOU, Li WANG, Yuan ZHAO, . Synthesis of artificial antigens of diethylstilbestrol and preparation of its antibody[J]. Frontiers of Agriculture in China, 2010 , 4(2) : 188 -194 . DOI: 10.1007/s11703-010-0097-8

Options
Outlines

/

〈 〉