Frontiers of Agriculture in China >
Cloning of strawberry FaEtr2 gene and its plant expression vector construction for antisense RNA
Received date: 19 Jul 2008
Accepted date: 17 Sep 2008
Published date: 05 Mar 2009
Copyright
An ethylene receptor FaEtr2 gene was amplified by Polymerase Chain Reaction (PCR) from ripening strawberry fruit. A 1049-bp PCR product (All Star-Etr2) was cloned. Sequence analysis showed that the All Star-Etr2 nucleotide sequence had 100% identity with Chandler-Etr2 from the GenBank. A pair of primers containing restriction enzyme sites were designed and used to amplify the sequenced plasmid. The PCR product was digested by the corresponding restricted enzymes and inserted between the CaMV 35S promoter and NOS terminator of expression vector pBI121 directionally. The constructed expression vector was transformed into Agrobacterium fumefeciens LBA4404 in the follow-up research to silence a ripening-related ethylene receptor FaEtr2 gene in strawberry fruits.
Key words： strawberry; ethylene receptor; FaEtr2 gene; plant expression vector
Chunli SONG , Pingping ZHOU , Junlian MA , Xia TANG , Zide ZHANG , Zhixia HOU . Cloning of strawberry FaEtr2 gene and its plant expression vector construction for antisense RNA[J]. Frontiers of Agriculture in China, 2009 , 3(1) : 55 -59 . DOI: 10.1007/s11703-009-0017-y
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