mRNA level of PKA-c gene in Setosphaeria turcica with different nutrition sources under metal ion or osmotic stress

Abstract

Intracellular signal transduction pathways including MAPK, Ca²⁺, and cAMP signal transduction pathways play important roles in regulating growth, development, and pathogenesis of phytopathogenic fungi. Protein kinase A (PKA) is a key enzyme in cAMP signaling pathway. The transcription level of the gene encoding catalytic subunit of PKA in Setosphaeria turcica under different culture conditions was analyzed by the semiquantitative RT-PCR method. The expression level of PKA-c gene was the lowest on the medium containing sucrose and starch as the carbon source, and it was distinctly inhibited by Cu²⁺, but it was independent of nitrogen source. After the addition of different concentrations of sorbitol, it showed the positive correlation between the inhibition affection and concentrations. However, the highest expression was observed in response to NaCl (0.9 mol/L). This research enriched the biological information resource of filamentous fungi and laid a foundation for the functional analysis about signal transduction pathway in phytopathogenic fungi.

Key words： Setosphaeria turcica; PKA; gene expression; semiquantitative RT-PCR

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