An efficient protocol for studying human pluripotent stem cell-derived myotube senescence

Qian Zhao; Ying Jing; Shuai Ma; Weiqi Zhang; Jing Qu; Si Wang; Guang-Hui Liu

doi:10.52601/bpr.2023.230013

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Biophysics Reports ›› 2023, Vol. 9 ›› Issue (5) : 232-240. DOI: 10.52601/bpr.2023.230013

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An efficient protocol for studying human pluripotent stem cell-derived myotube senescence

Qian Zhao1,2 ,
Ying Jing1,2 ,
Shuai Ma4,6,8 ,
Weiqi Zhang5,7,8,9,10 ,
Jing Qu3,5,6,8 ,
Si Wang1,2 ,
Guang-Hui Liu1,2,4,5,6,8

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Abstract

Sarcopenia, an age-related skeletal muscle condition characterized by a progressive decline in muscle mass and function, is linked to increased vulnerability, a higher likelihood of falls, and higher mortality rates in older individuals. A comprehensive understanding of the intricate mechanisms driving skeletal muscle aging is of great significance in both scientific and clinical fields. Consequently, myotube models that facilitate studying regulatory mechanisms underlying skeletal muscle aging are important tools required to advance intervention strategies against skeletal muscle aging and associated disorders. Here, we provide a detailed protocol to generate human pluripotent stem cells-derived myotubes and describe their applications in aging studies, as well as a troubleshooting for potential problems. Overall, this protocol serves as a valuable methodological reference for exploring the role and mechanism of genes involved in skeletal muscle aging.

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Keywords

Human pluripotent stem cell / Myotube / Senescence / Differentiation / In vitro

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Qian Zhao, Ying Jing, Shuai Ma, Weiqi Zhang, Jing Qu, Si Wang, Guang-Hui Liu. An efficient protocol for studying human pluripotent stem cell-derived myotube senescence. Biophysics Reports, 2023, 9(5): 232‒240 https://doi.org/10.52601/bpr.2023.230013

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Acknowledgements

We greatly thank Xiuping Li, Jing Lu, Ying Yang, and Lei Bai for their administrative assistance. This work was supported by the National Natural Science Foundation of China (82071588, 82122024), the National Key Research and Development Program of China (2022YFA1103700, 2020YFA0804000, 2020YFA0112200, 2021YFF1201000, the STI2030-Major Projects-2021ZD0202400), the National Natural Science Foundation of China (82001477, 81921006, 82125011, 92149301, 92168201, 91949209, 92049304, 92049116, 32121001, 82192863, 82122024, 32000500, 82271600), CAS Project for Young Scientists in Basic Research (YSBR-076, YSBR-012), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16000000), the Program of the Beijing Natural Science Foundation (Z190019), the Informatization Plan of Chinese Academy of Sciences (CAS-WX2021SF-0301, CAS-WX2022SDC-XK14, CAS-WX2021SF-0101), New Cornerstone Science Foundation through the XPLORER PRIZE (2021-1045), Youth Innovation Promotion Association of CAS (E1CAZW0401, 2022083), Excellent Young Talents Program of Capital Medical University (12300927), The Project for Technology Development of Beijing-affiliated Medical Research Institutes (11000023T000002036310), Excellent Young Talents Training Program for the Construction of Beijing Municipal University Teacher Team (BPHR202203105), Young Elite Scientists Sponsorship Program by CAST (YESS20210002), and Beijing Hospitals Authority Youth Program (QML20230806).