THE NEW OPPORTUNITIES OF HUMAN EMBRYO CULTIVATING IN VITRO
O. V. Shurygina , V. K. Belyakov , G. B. Nemkovskiy , A. B. Kuznezov , O. V. Ivanova , M. T. Tugushev , O. V. Kulakova
Morphology ›› 2020, Vol. 157 ›› Issue (1) : 75 -78.
THE NEW OPPORTUNITIES OF HUMAN EMBRYO CULTIVATING IN VITRO
Objective - comparative analysis of the effectiveness of time-lapse technology and the standard cultivation of human embryos in vitro. Material and methods. A study of 465 developing human embryos using time-lapse technology was conducted. Morphokinetic parameters (the time intervals of the first cleavage, the presence/absence of the reverse cleavage, the direct division of the zygote into three blastomeres) and intracellular changes (multinucleation, fragmentation, vacuolization, etc.) were evaluated. The control group consisted of 512 embryos of preimplantation development using a standard cultivation technique. Standard key indicators of developing embryos (fertilization, growth to blastocysts, freezing, utilization rate, clinical pregnancy rate) were used as the criteria for assessing efficiency. Results. The key indicators of pre-implantation development of embryos did not show a significant difference in the time-lapse monitored group and the control. However, the blastocyst development rate, the freezing rate, the embryo utilization rate in the time-lapse group were slightly higher, as well as the pregnancy clinical rate of 42,6 versus 38,5 %. Conclusions. The time-lapse technology allows to reduce the adverse effects of environmental factors, increasing the cultivation conditions in vitro. Registration of key morphodynamic events and their analysis make it possible to more objectively evaluate the pre-implantation development of human embryos and select the most promising embryo for implantation for transfer.
embryo / cultivation / time-lapse monitoring / human
Shurygina O.V., Belyakov V.K., Nemkovskiy G.B., Kuznezov A.B., Ivanova O.V., Tugushev M.T., Kulakova O.V.
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