METHOD OF SELECTIVE DEMONSTRATION OF PROLIFERATING CELLS IN THE STRUCTURES OF DEVELOPING NERVOUS SYSTEM BASED ON THE DETECTION OF PHOSPHORYLATED H3 HISTONE
D. E. Korzhevskiy , O. V. Kirik , G. V. Beznin
Morphology ›› 2012, Vol. 142 ›› Issue (6) : 069 -072.
METHOD OF SELECTIVE DEMONSTRATION OF PROLIFERATING CELLS IN THE STRUCTURES OF DEVELOPING NERVOUS SYSTEM BASED ON THE DETECTION OF PHOSPHORYLATED H3 HISTONE
Phosphorylated H3 histone is of interest for the study of proliferative activity of the cells in various tissues. Its detection is used in histopathological tumor diagnosis. The purpose of this study was to develop immunocytochemical protocols for the demonstration of H3phosphohistone in the cells of the developing nervous system for classical light microscopy and confocal laser microscopy. The protocols presented allow to identify the dividing cells in the structures of developing brain with high selectivity and good reproducibility. Zinc-ethanol-formaldehyde is recommended as an optimal fixative for H3phosphohistone.
brain / H3 histone / cell proliferation / immunocytochemistry
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