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Frontiers in Biology

Front. Biol.    2016, Vol. 11 Issue (4) : 305-310     DOI: 10.1007/s11515-016-1408-0
Cytogenetic changes of mesenchymal stem cells in the neoplastic bone marrow niche in leukemia
Shirin Ferdowsi1,Shirin Azizidoost2,Nasim Ghafari3,Najmaldin Saki2,3,*()
1. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2. Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3. Golestan Hospital Clinical Research Development Unit, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
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BACKGROUND: Bone marrow mesenchymal stromal cells (BM-MSCs) are an essential cell type in the hematopoietic microenvironment. The question of whether MSCs from patients with different leukemias have cytogenetic abnormalities is controversial. In this study, we attempted to review the cytogenetic profiles of MSCs in patients with leukemia, and verify whether these profiles were related to different ex vivo culture conditions or to chronic or acute disease states. This information could be useful in clarifying the origin of MSCs and developing clinical applications for this cell type.

METHODS: A systematic literature search was performed using the PubMed search engine. Studies published over the past 15 years, i.e., between 1995 and January 2015, were considered for review. The following keywords were used: “cytogenetic,” “leukemia,” “bone marrow,” and “mesenchymal stromal cells.”

RESULTS: Some studies demonstrated that BM-MSCs are cytogenetically normal, whereas others provided evidence of aberrations in these cells.

CONCLUSIONS: Studying cytogenetic changes of MSCs in a variety of leukemias will help researchers understand the nature of these tumors and ensure the safety of human stem cells in clinical applications.

Keywords bone marrow      mesenchymal stromal cells      leukemia      cytogenetic      niche     
Corresponding Authors: Najmaldin Saki   
Just Accepted Date: 21 June 2016   Online First Date: 18 July 2016    Issue Date: 30 August 2016
 Cite this article:   
Shirin Ferdowsi,Shirin Azizidoost,Nasim Ghafari, et al. Cytogenetic changes of mesenchymal stem cells in the neoplastic bone marrow niche in leukemia[J]. Front. Biol., 2016, 11(4): 305-310.
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Shirin Ferdowsi
Shirin Azizidoost
Nasim Ghafari
Najmaldin Saki
Diagnosis Cytogenetic markers in MSCs Molecular change Reference
AML •t(1;2)(p32;q31), t(1;6)(p32;p12), del(7)(q11.2q32), del(7)(q22), t(3;20)(p13;p11.2), del(11)(q23), -X, del(13)(q12q22), inv(X)(q12p22), +5, -4, -X, -Y•t(2;11)(q33;q13), del(4)(q12q21), der(5;17)(p10;q10), del(7) (q22q34),-6, +8,+16,-18,-20,-22, dd(5)(q13)•t(1;10)(p36;p12), t(7;9)(q11.2;q34), t(7;10)(q11.2;q21), del(3)(p21), del(11)(q23),-22, del(8)(q11), +13, t(2;13),-12•t(3;9)(q25;q31), del(1)(q42) ↓ MCP-1,GM-CSF,IL-6 ↑ FOS,MYB Blau et al., 2007; Blau et al., 2011; Yeh et al., 2012; Huang et al., 2015
MDS •del(7)(q22), inv(X)(q12p22),+5, -4, -Y•der(7)t(1;7), del(17)(p11.2), t(4;7)(p14;q22), dic(6;16), -16,-17, t(7;19)(q22;q13), t(15;17)(q26;q12), t(1;3)(p12;q13), del(2)(q31), ?????↑ AURKA Blau et al., 2007, 2011
Pro-B-All •t(4;11) MLL-AF4 - - - - Menendez et al., 2009
CLL •Normal - - - - Campioni et al., 2012
ALL •Normal& der(13;14)(q10;q10) - - - - Campioni et al., 2012; Yeh et al., 2012
MPN •Loss 7pter-p22.2, loss 7p21.3, loss 7p21.3-p15.2, loss 7p12.3-p12.1, loss 7q11.22, gain 7q11.23-qter, Gain 7q22.1-qter, 5+, 7+, Loss 11q13.2-q13.4, loss 1q42.11-q44, loss 3p21.31-p11.1, loss 17q11.1-q11.2•Normal for JAK2V617F positive ↓G-CSF, IL-7 Pieri et al., 2008; Mercier et al., 2009; Avanzini et al., 2014
CML •Normal for t(9;22) - - - - Jootar et al., 2006; Carrara et al., 2007; Wöhrer et al., 2007
MM •Normal & Chromosomal loss at 4p14-4p13 and 3q13.13 ↑ AREG, DKK1, IL-1β↓IGF-1, SDF-1 Garayoa et al., 2009
Tab.1  Cytogenetic aberrations in BM-MSCs from leukemia patients.
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