Please wait a minute...

Frontiers of Environmental Science & Engineering

Front.Environ.Sci.Eng.    2008, Vol. 2 Issue (1) : 116-121     https://doi.org/10.1007/s11783-008-0018-8
|
Microbial community structure in different wastewater treatment processes characterized by single-strand conformation polymorphism (SSCP) technique
ZHAO Yangguo1, WANG Aijie2, REN Nanqi2, ZHAO Yan2
1.School of Municipal and Environmental Engineering, Harbin Institute of Technology; College of Environmental Science and Engineering, Ocean University of China; 2.School of Municipal and Environmental Engineering, Harbin Institute of Technology;
Download: PDF(331 KB)   HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract In order to investigate microbial community structures in different wastewater treatment processes and understand the relationship between the structures and the status of processes, the microbial community diversity, variety and distribution in five wastewater treatment pro cesses were studied by a culture-independent genetic fingerprinting technique single-strand conformation polymorphism (SSCP). The five processes included denitrifying and phosphate-removal system (diminished N), Chinese traditional medicine wastewater treatment system (P), beer wastewater treatment system (W), fermentative biohydrogen-producing system (H), and sulfate-reduction system (S). The results indicated that the microbial community profiles in the wastewater bioreactors with the uniform status were very similar. The diversity of microbial populations was correlated with the complexity of organic contaminants in wastewater. Chinese traditional medicine wastewater contained more complex organic components; hence, the population diversity was higher than that of simple nutrient bioreactors fed with molasses wastewater. Compared with the strain bands in a simulated community, the relative proportion of some functional microbial populations in bioreactors was not dominant. Fermentative biohydrogen producer Ethanoligenens harbinense in the better condition bioreactor had only a 5% band density, and the Desulfovibrio sp. in the sulfate-reducing bioreactor had less than 1.5% band density. The SSCP profiles could identify the difference in microbial community structures in wastewater treatment processes, monitor some of the functional microbes in these processes, and consequently provide useful guidance for improving their efficiency.
Issue Date: 05 March 2008
 Cite this article:   
WANG Aijie,ZHAO Yangguo,REN Nanqi, et al. Microbial community structure in different wastewater treatment processes characterized by single-strand conformation polymorphism (SSCP) technique[J]. Front.Environ.Sci.Eng., 2008, 2(1): 116-121.
 URL:  
http://journal.hep.com.cn/fese/EN/10.1007/s11783-008-0018-8
http://journal.hep.com.cn/fese/EN/Y2008/V2/I1/116
Service
E-mail this article
E-mail Alert
RSS
Articles by authors
WANG Aijie
ZHAO Yangguo
REN Nanqi
ZHAO Yan
1 Burrell P C Keller J Blackall L L Microbiology of a nitrite-oxidizing bioreactorAppl Environ Microbiol 1998 641,8781,883
2 Fernandez A S Hashsham S A Dolhopf S L Criddle C S Tiedje J M Flexible community structure correlateswith stable community function in methanogenic bioreactor communitiesperturbed by glucoseAppl Environ Microbiol 2000 664,0584,067
3 Liu W T Marsh T L Cheng H Forney L J Characterizationof microbial diversity by determining terminal restriction fragmentlength polymorphisms of genes encoding 16S rRNAAppl Environ Microbiol 1997 634,5164,522
4 Muyzer G De Waal E C Uitterlinden A G Profiling of complex microbial populations by denaturinggradient gel electrophoresis analysis of polymerase chain reaction-amplifiedgenes coding for 16S rRNAAppl Environ Microbiol 1993 59695700
5 Schwieger F Tebbe C C A new approach to utilize PCR–singlestrand-conformation polymorphism for 16S rRNA gene-based microbialcommunity analysisAppl Environ Microbiol 1998 644,8704,876
6 Lee D H Zo Y G Kim S J Nonradioactive method to study genetic profiles of naturalbacterial communities by PCR-single-strand-conformation polymorphismAppl Environ Microbiol 1996 623,1123,120
7 Schmalenberger A Tebbe C C Bacterial diversity in maizerhizospheres: Conclusions on the use of genetic profiles based onPCR-amplified partial small subunit rRNA genes in ecological studiesMol Ecol 2003 12251262.
doi:10.1046/j.1365‐294X.2003.01716.x
8 Sliwinski M K Goodman R M Spatial heterogeneity of crenarchaealassemblages within mesophilic soil ecosystems as revealed by PCR-single-strandedconformation polymorphism profilingApplEnviron Microbiol 2004 701,8111,820
9 Delbàs C Moletta R Godon J J Bacterial and archaeal 16S rDNA and 16S rRNA dynamics duringan acetate crisis in an anaerobic digestor ecosystemFEMS Microbiol Ecol 2001 351626
10 Zumstein E Moletta R Godon J J Examination of two years of community dynamics in an anaerobicbioreactor using fluorescence polymerase chain reaction (PCR) single-strandconformation polymorphism analysisEnvironMicrobiol 2000 26978.
doi:10.1046/j.1462‐2920.2000.00072.x
11 American Public HealthAssociation (APHA). . Standard Methods for the Examinationof Water and Wastewater, 20th edWashington,USAAmerican Public Health Association 1998
12 Ren N Q Wang B Z Huang J C Ethanol-type fermentation from carbohydrate in high rateacidogenic reactorBiotechnol Bioeng 1997 54428433.
doi:10.1002/(SICI)1097‐0290(19970605)54:5<428::AID‐BIT3>3.0.CO;2‐G
13 Bassam B J Caetano-Anolles G Gresshoff P M Fast and sensitive silver staining of DNA in polyacrylamidegelsAnal Biochem 1991 1968083.
doi:10.1016/0003‐2697(91)90120‐I
14 Stach J E M Bathe S Clapp J P Burn R G PCR-SSCP comparisonof 16S rDNA sequence diversity in soil DNA obtained using differentisolation and purification methodsFEMSMicrobiol Ecol 2001 36139151.
doi:10.1111/j.1574‐6941.2001.tb00834.x
15 Miller D N Bryant J E Madsen E L Ghiorse W C Evaluationand optimization of DNA extraction and purification procedures forsoil and sediment samplesAppl Environ Microbiol 1999 654,7154,724
16 Dar S A Kuenen J G Muyzer G Nested PCR-denaturing gradient gel electrophoresis approachto determine the diversity of sulfate-reducing bacteria in complexmicrobial communitiesAppl Environ Microbiol 2005 712,3252,330.
doi:10.1128/AEM.71.5.2325‐2330.2005
17 Zhao Y G Ren N Q Wang A J Liu G M Zhao Q S Shang H X Microbialcommunity structure analyzed by single-strand conformation polymorphismtechnique in sulfate-reducing reactorChin.J Environ Sci 2005 26(4)171176(in Chinese)
18 Shi Y Ren N Q Yan X F Ding J Wang J Q Gao Y Full-scalestudies of biological treatment of wastewater from traditional Chinesemedicine productionJ Dalian Univ Technol 2003 43(7)438441(in Chinese)
19 Ahn J Daidou T Tsuneda S Hirata A Metabolic behaviorof denitrifying phosphate-accumulating organisms under nitrate andnitrite electron acceptor conditionsJ BiosciBioeng 2001 92442446.
doi:10.1263/jbb.92.442
20 Kishida N Kim J Tsuneda S Sudo R Anaerobic/oxic/anoxicgranular sludge process as an effective nutrient removal process utilizingdenitrifying polyphosphate-accumulating organismsWater Res 2006 402,3032,310.
doi:10.1016/j.watres.2006.04.037
21 Tsuneda S Miyauchi R Ohno T Hirata A Characterizationof denitrifying polyphosphate-accumulating organisms in activatedsludge based on nitrite reductase geneJBiosci Bioeng 2005 99403407.
doi:10.1263/jbb.99.403
22 Ren N Q Xing D F Rittmann B E Zhao L H Xie T H Zhao X Microbialcommunity structure of ethanol type fermentation in bio-hydrogen productionEnviron Microbiol 2007 91,1121,125.
doi:10.1111/j.1462‐2920.2006.01234.x
23 Zhao Y G Ren N Q Wang A J Shang H X Characterizationof efficiency-enhancing bacterium for sulfate wastewater treatmentand structure analysis of dissimilatory sulfite reductase geneJ Chem Ind Engin 2006 572,4012,406(in Chinese)
24 Liu G M Ren N Q Wang A J Wang X J Du D Z Chen M Q Thefermentation type of acidogenic bacteria and their cooperation withSRB in an acidogenic sulfate-reducing reactorActa Scientiae Circumstantiae 2004 24782788(in Chinese)
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed