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Frontiers of Environmental Science & Engineering

Front. Environ. Sci. Eng.    2017, Vol. 11 Issue (6) : 16     https://doi.org/10.1007/s11783-017-0964-0
RESEARCH ARTICLE |
Impact of dissolved oxygen on the production of nitrous oxide in biological aerated filters
Qiang He, Yinying Zhu, Guo Li, Leilei Fan, Hainan Ai, Xiaoliu Huangfu, Hong Li()
Key Laboratory of Eco-Environment of Three Gorges Region, Ministry of Education, Chongqing University, Chongqing 400045, China
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Abstract

The dominant Cloacibacterium normanense may be responsible for N2O production.

N2O concentrations varied along the biofilm depth depending on the DO levels.

Low DO concentration leads to high N2O production rate.

Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and microelectrode technology were employed to evaluate the Nitrous oxide (N2O) production in biological aerated filters (BAFs) under varied dissolved oxygen (DO) concentrations during treating wastewater under laboratory scale. The average yield of gasous N2O showed more than 4-fold increase when the DO levels were reduced from 6.0 to 2.0 mg·L1, indicating that low DO may drive N2O generation. PCR-DGGE results revealed that Nitratifractor salsuginis were dominant and may be responsible for N2O emission from the BAFs system. While at a low DO concentration (2.0 mg·L1), Flavobacterium urocaniciphilum might play a role. When DO concentration was the limiting factor (reduced from 6.0 to 2.0 mg·L1) for nitrification, it reduced NO2-N oxidation as well as the total nitrification. The data from this study contribute to explain how N2O production changes in response to DO concentration, and may be helpful for reduction of N2O through regulation of DO levels.

Keywords Nitrous oxide      Biological aerated filter      Microelectrode      Dissolved oxygen      Biofilm     
Corresponding Authors: Hong Li   
Issue Date: 10 July 2017
 Cite this article:   
Qiang He,Yinying Zhu,Guo Li, et al. Impact of dissolved oxygen on the production of nitrous oxide in biological aerated filters[J]. Front. Environ. Sci. Eng., 2017, 11(6): 16.
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http://journal.hep.com.cn/fese/EN/10.1007/s11783-017-0964-0
http://journal.hep.com.cn/fese/EN/Y2017/V11/I6/16
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Qiang He
Yinying Zhu
Guo Li
Leilei Fan
Hainan Ai
Xiaoliu Huangfu
Hong Li
Fig.1  Simulated BAF system in this study. a, plexiglass tank; b, flowrator (MP200-YZ15, PreFluid, Changzhou, China); c, flowrator (LZB-2, Variable-area Flowmeter, Shanghai, China); d, air pump (WT-ZWS24Z, China) and e, flowrator (Q-Flow, Zhuoang, Shanghai, China)
Fig.2  COD (a), TN (b), NH4-N (c), NO3--N (d), and NO2--N (e) concentration in the influent and effluent of BAF systems exposed at different DO levels, pH 7.2, 20℃
Fig.3  Effect of DO on the production rate of N2O, pH 7.2, 20℃
time/dtreatment (DO level)
DO= 2.0 mg·L-1DO= 4.0 mg·L-1DO= 6.0 mg·L-1
31.971.330.51
61.360.890.48
91.700.880.41
122.571.330.71
151.740.800.37
181.970.560.33
211.150.420.24
241.790.480.25
271.750.650.28
Tab.1  Dynamic of N2O emission factors (N2O /TNinfluent) under varied DO levels
Fig.4  DGGE profile of 16S rRNA gene fragments of microbial communities from BAF biofilm under different DO levels, pH 7.2, 20℃
numbermost similar strainaccession numbersemblancemost similar groups
band 1Caulobacter daechungensisNR_118485100Proteobacteria
band 2Cloacibacterium normanenseNR_04218799Bacteroidetes
band 3Lutibacter aestuariiNR_10899590Bacteroidetes
band 4Acidovorax radicisNR_117776100Proteobacteria
band 5Epistylis urceolataAF33551698Alveolata
band 6Nitratifractor salsuginisNR_07443088Proteobacteria
band 7Crenotalea thermophilaNR_12547391Bacteroidetes
band 8Ferruginibacter alkalilentusNR_04458897Bacteroidetes
band 9Peptoniphilus lacrimalisNR_04193884Firmicutes
band 10Cloacibacterium normanenseNR_042187100Bacteroidetes
band 11Ottowia shaoguanensisNR_12565698Proteobacteria
band 12Flavobacterium ginsengisoliNR_10902498Bacteroidetes
band 13Clostridium cellulovoransNR_02758988Firmicutes
band 14Clostridium acidisoliNR_028898100Firmicutes
band 15Kofleria flavaNR_04198189Proteobacteria
band 16Tahibacter aquaticusNR_11509895Proteobacteria
band 17Desulfobulbus rhabdoformisNR_02917696Proteobacteria
band 18Thiothrix caldifontisNR_116398100Proteobacteria
band 19Flavobacterium urocaniciphilumNR_12546791Bacteroidetes
band 20Methylocaldum marinumNR_12618992Proteobacteria
band 21Thiobacillus thioparusNR_11786495Proteobacteria
band 22Blastocatella fastidiosaNR_11835096Acidobacteria
Tab.2  Results of 16S rDNA sequences using BLAST in GeneBank
Fig.5  Changes in concentrations of N2O with depth in biofilm, pH 7.2, 20℃
Fig.6  Changes in DO concentrations (a), ORP value (b), NH4+-N concentrations (c), NO2--N concentrations (d), NO3--N concentrations (e) with depth in biofilm, pH 7.2, 20℃
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