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Frontiers of Agricultural Science and Engineering

Front. Agr. Sci. Eng.    2016, Vol. 3 Issue (3) : 186-194     https://doi.org/10.15302/J-FASE-2016107
REVIEW |
Molecular regulation and genetic improvement of seed oil content in Brassica napus L.
Wei HUA,Jing LIU,Hanzhong WANG()
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
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Abstract

As an important oil crop and a potential bioenergy crop, Brassica napus L. is becoming a model plant for basic research on seed lipid biosynthesis as well as seed oil content, which has always been the key breeding objective. In this review, we present current progress in understanding of the regulation of oil content in B. napus, including genetics, biosynthesis pathway, transcriptional regulation, maternal effects and QTL analysis. Furthermore, the history of breeding for high oil content in B. napus is summarized and the progress in breeding ultra-high oil content lines is described. Finally, prospects for breeding high oil content B. napus cultivars are outlined.

Keywords breeding      maternal effects      oilseed rape      QTL     
Corresponding Authors: Hanzhong WANG   
Just Accepted Date: 18 July 2016   Online First Date: 02 August 2016    Issue Date: 21 September 2016
 Cite this article:   
Wei HUA,Jing LIU,Hanzhong WANG. Molecular regulation and genetic improvement of seed oil content in Brassica napus L.[J]. Front. Agr. Sci. Eng. , 2016, 3(3): 186-194.
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http://journal.hep.com.cn/fase/EN/10.15302/J-FASE-2016107
http://journal.hep.com.cn/fase/EN/Y2016/V3/I3/186
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Wei HUA
Jing LIU
Hanzhong WANG
Organ Function Gene Specie Reference No.
Embryo Fatty acid synthesis Acetyl-CoA carboxylase (ACCase) Arabidopsis [13]
TAG synthesis Acyl-CoA:sn-glycerol-3-phosphate acyltransferase (GPAT) Arabidopsis [19]
sn-Glycerol-3-phosphate dehydrogenase (G3PDH) Rapeseed [14]
Acyl-CoA:lysophosphatidic acid acyltransferase (LPAAT) Arabidopsis, rapeseed [20,21]
Type 1 acyl-CoA: diacylglycerol acyltransferase (DGAT1) Arabidopsis, rapeseed, maize [15,16]
Type 2 acyl-CoA: diacylglycerol acyltransferase (DGAT2) Soybean [18]
Glycolysis related Mitochondrial pyruvate dehydrogenase kinase (PDHK) Arabidopsis, rapeseed [26,27]
Cytosolic D-glucose-6-phosphate dehydrogenase (Glu6PDH) Arabidopsis [28]
Plastidial heteromeric pyuvate kinase complex Arabidopsis [25]
Transcription regulation Leafy contyledon 1 (LEC1) Arabidopsis, rapeseed, maize [34,35,37]
Leafy contyledon 2 (LEC2) Arabidopsis [36]
Transparent tetsa 2(TT2) Arabidopsis [40]
Wrinkled 1(WRI1) Arabidopsis, rapeseed, maize [30,32,37]
Seed coat Seed size regulating genes in embryo Apetala 2 (AP2) Arabidopsis [49]
CYP78A5 (KLU) Arabidopsis [48]
Transparent testa 8 (TT8) Arabidopsis [53]
Mother plant Photosynthesis Growth-regulating factor 2 (GRF2) Rapeseed [51]
All Cytoplast effect ORF188, a mitochondrial gene Rapeseed [55]
Tab.1  Identification and function of genes affecting seed oil content
Fig.1   Regulation model of seed oil content in Brassica napus. Boldfaces indicate major organs or factors controlling the seed oil content and their relative regulating pathways are listed in the parenthesis.
Oil content of parents/% Localization method QTL number Position Contribution/% Reference No.
43.6, 42 DHP, ML 7 N1,N3,N4,N8,N12,N13,N17 2.4–15.7 [58]
48, 46 DHP, ML 15 N1, N2, N3, N5, N6, N10, N12, N13, N15, N16, N17 1.2–13.4 [59]
47.5, 41.7 DHP, ML 8 N1, N4, N6, N12, N16, N17, N19 1.77–27.57 [59]
43.28, 37.03 DHP, ML 5 N1, N8, N10, N13 5.21–10.17 [64]
39.7, 34.8 DHP, ML 7 N4, N7, N11, N16, N17 3.73–10.46 [63]
39.70, 34.80 DHP, ML 9 N1, N3, N4, N5, N7, N13, N14 5.19–13.57 [65]
N/A DHP, ML 19 N1,N2,N3,N4,N5,N6,N7,N8,N10,N12,N13,N14,N16, N18 4.2–30.2 [66]
49.53, 39.42 DHP, ML 12 A2, A3, A5, A6, C2, C5, C8, C9 9.15–24.56 [69]
30–52 NP, GWAS 12 A1, A3, A9, A10, C2, C3 3–15 [67]
NP, GWAS 26 A1, A3, A4, A9, A10, C2, C3 5–15 [67]
32.66–46.73 NP, GWAS 4 A1, A5, A7, A8 4.42–13.13 [68]
34.2–51.4 NP, GWAS 1 A8 6.22 [74]
Tab.2  QTLs affecting seed oil content in B. napus
1 Shang G X. Study on inheritance and NIRS model establishing of high oleic acid content in Brassica napus L. Dissertation for the Master Degree. Chongqing: Southwestern University, 2010 (in Chinese)
2 Wang H Z. Review and future development of rapeseed industry in China. Chinese Journal of Oil Crop Sciences, 2010, 32(2): 300–302 (in Chinese)
3 Li Y C, Hu Q, Mei D S, Li Y D, Xu Y S. Theory and practice for the development of canola varieties with high oil content. Chinese Journal of Oil Crop Sciences, 2006, 28(1): 92–96 (in Chinese)
4 Wang H Z. Studies on microspore culture of hybrid parents in Brassica napus. Chinese Journal of Oil Crop Sciences, 2004, 26(1): 98–101 (in Chinese)
5 Hu Z Y, Hua W, Zhang L, Deng L B, Wang X F, Liu G H, Hao W J, Wang H Z. Seed structure characteristics to form ultrahigh oil content in rapeseed. PLoS ONE, 2013, 8(4): e62099
https://doi.org/10.1371/journal.pone.0062099
6 Gan G X, Lin S C. Rapeseed oil content and high oil content breeding. Seed, 1997, 1: 31–33
7 Wang T. Genetic and heterosis of rapeseed oil content. Journal of Guizhou Agricultural Sciences, 1992, 6: 37–42 (in Chinese)
8 Wu J G, Shi C H, Zhang H Z. Partitioning genetic effects due to embryo,cytoplasm and maternal parent for oil content in oilseed rape (Brassica napus L.). Genetics and Molecular Biology, 2006, 29(3): 533–538
https://doi.org/10.1590/S1415-47572006000300023
9 Wang X F, Liu G H, Yang Q, Hua W, Liu J, Wang H Z. Genetic analysis on oil content in rapeseed Brassica napus L. Euphytica, 2010, 173(1): 17–24
https://doi.org/10.1007/s10681-009-0062-x
10 Weselake R J. Storage lipids. In: Murphy D J, editor. Plant lipids: biology, utilization and manipulation. Oxford: Blackwell Publishing, 2005, 162–221
11 Lung S C, Weselake R J. Diacylglycerol acyltransferase: a key mediator of plant triacylglycerol synthesis. Lipids, 2006, 41(12): 1073–1088
https://doi.org/10.1007/s11745-006-5057-y
12 Snyder C L, Yurchenko O P, Siloto R M, Chen X, Liu Q, Mietkiewska E, Weselake R J. Acyltransferase action in the modification of seed oil biosynthesis. New Biotechnology, 2009, 26(1–2): 11–16
https://doi.org/10.1016/j.nbt.2009.05.005
13 Roesler K, Shintani D, Savage L, Boddupalli S, Ohlrogge J. Targeting of the Arabidopsis homomeric acetyl-coenzyme A carboxylase to plastids of rapeseeds. Plant Physiology, 1997, 113(1): 75–81
https://doi.org/10.1104/pp.113.1.75
14 Vigeolas H, Waldeck P, Zank T, Geigenberger P. Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter. Plant Biotechnology Journal, 2007, 5(3): 431–441
https://doi.org/10.1111/j.1467-7652.2007.00252.x
15 Jako C, Kumar A, Wei Y, Zou J, Barton D L, Giblin E M, Covello P S, Taylor D C. Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight. Plant Physiology, 2001, 126(2): 861–874
https://doi.org/10.1104/pp.126.2.861
16 Taylor D C, Zhang Y, Kumar A, Francis T, Giblin E M, Barton D L, Ferrie J R, Laroche A, Shah S, Zhu W, Snyder C L, Hall L, Rakow G, Harwood J L, Weselake R J. Molecular modification of triacylglycerol accumulation by over-expression of DGAT1 to produce canola with increased seed oil content under field conditions. Botany, 2009, 87(6): 533–543
https://doi.org/10.1139/B08-101
17 Zheng P, Allen W B, Roesler K, Williams M E, Zhang S, Li J, Glassman K, Ranch J, Nubel D, Solawetz W, Bhattramakki D, Llaca V, Deschamps S, Zhong G Y, Tarczynski M C, Shen B. A phenyalanine in DGAT is a key determinant of oil content and composition in maize. Nature Genetics, 2008, 40(3): 367–372
https://doi.org/10.1038/ng.85
18 Lardizabal K D, Effertz R, Levering C, Mai J, Pedroso M C, Jury T, Aasen E, Gruys K, Bennett K. Expression of Umbelopsis ramanniana DGAT2A in seed increases oil in soybean. Plant Physiology, 2008, 148(1): 89–96
https://doi.org/10.1104/pp.108.123042
19 Jain R K, Coffey M, Lai K, Kumar A, MacKenzie S L. Enhancement of seed oil content by expression of glycerol-3-phosphate acyltransferase genes. Biochemical Society Transactions, 2000, 28(6): 958–961
https://doi.org/10.1042/bst0280958
20 Zou J T, Katavic V, Giblin E M, Barton D L, MacKenzie S L, Keller W A, Hu X, Taylor D C. Modification of seed oil content and acyl composition in Brassicaceae by expression of a yeast sn-2 acyltransferase gene. Plant Cell, 1997, 9(6): 909–923
https://doi.org/10.1105/tpc.9.6.909
21 Taylor D C, Katavic V, Zou J, MacKenzie S L, Keller W A, An J, Friesen W, Barton D L, Pedersen K K, Michael Giblin E, Ge Y, Dauk M, Sonntag C, Luciw T, Males D. Field-testing of transgenic rapeseed cv. Hero transformed with a yeast sn-2 acyltransferase results in increased oil content, erucic acid content and seed yield. Molecular Breeding, 2001, 8(4): 317–322
https://doi.org/10.1023/A:1015234401080
22 Weselake R J, Taylor D C, Rahman M H, Shah S, Laroche A, McVetty P B, Harwood J L. Increasing the flow of carbon into seed oil. Biotechnology Advances, 2009, 27(6): 866–878
https://doi.org/10.1016/j.biotechadv.2009.07.001
23 Xu Y L, Guan C Y, Tan T L, Yu L X. Changes of oil content and oil biosynthesis-related enzymes activities and their correlation during seed formation in Brassica napus. Acta Agronomica Sinica, 2008, 34(10): 1854–1857 (in Chinese)
https://doi.org/10.3724/SP.J.1006.2008.01854
24 Plaxton W C, Podesta F E. The functional organization and control of plant respiration. Critical Reviews in Plant Sciences, 2006, 25(2): 159–198
https://doi.org/10.1080/07352680600563876
25 Andre C, Froehlich J E, Moll M R, Benning C. A heteromeric plastidic pyruvate kinase complex involved in seed oil biosynthesis in Arabidopsis. Plant Cell, 2007, 19(6): 2006–2022
https://doi.org/10.1105/tpc.106.048629
26 Zou J T, Qi Q, Katavic V, Marillia E F, Taylor D C. Effects of antisense repression of an Arabidopsis thaliana pyruvate dehydrogenase kinase cDNA on plant development. Plant Molecular Biology, 1999, 41(6): 837–849
https://doi.org/10.1023/A:1006393726018
27 Marillia E F, Micallef B J, Micallef M, Weninger A, Pedersen K K, Zou J, Taylor D C. Biochemical and physiological studies of Arabidopsis thaliana transgenic lines with repressed expression of the mitochondrial pyruvate dehydrogenase kinase. Journal of Experimental Botany, 2003, 54(381): 259–270
https://doi.org/10.1093/jxb/erg020
28 Wakao S, Andre C, Benning C. Functional analyses of cytosolic glucose-6-phosphate dehydrogenases and their contribution to seed oil accumulation in Arabidopsis. Plant Physiology, 2008, 146(1): 277–288
https://doi.org/10.1104/pp.107.108423
29 Santos-Mendoza M, Dubreucq B, Baud S, Parcy F, Caboche M, Lepiniec L. Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis. Plant Journal, 2008, 54(4): 608–620
https://doi.org/10.1111/j.1365-313X.2008.03461.x
30 Focks N, Benning C. Wrinkled1: a novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism. Plant Physiology, 1998, 118(1): 91–101
https://doi.org/10.1104/pp.118.1.91
31 Baud S Ã, Wuillème S, To A, Rochat C, Lepiniec L Ã. Role of WRINKLED1 in the transcriptional regulation of glycolytic and fatty acid biosynthetic genes in Arabidopsis. Plant Journal, 2009, 60(6): 933–947
https://doi.org/10.1111/j.1365-313X.2009.04011.x
32 Liu J, Hua W, Zhan G, Wei F, Wang X, Liu G, Wang H. Increasing seed mass and oil content in transgenic Arabidopsis by the overexpression of wri1-like gene from Brassica napus. Plant Physiology and Biochemistry, 2010, 48(1): 9–15
https://doi.org/10.1016/j.plaphy.2009.09.007
33 Lotan T, Ohto M, Yee K M, West M A L, Lo R, Kwong R W, Yamagishi K, Fischer R L, Goldberg R B, Harada J J. Arabidopsis LEAFY COTYLEDON1 is sufficient to induce embryo development in vegetative cells. Cell, 1998, 93(7): 1195–1205
https://doi.org/10.1016/S0092-8674(00)81463-4
34 Mu J, Tan H, Zheng Q, Fu F, Liang Y, Zhang J, Yang X, Wang T, Chong K, Wang X, Zuo J. LEAFY COTYLEDON1 is a key regulator of fatty acid biosynthesis in Arabidopsis. Plant Physiology, 2008, 148(2): 1042–1054
https://doi.org/10.1104/pp.108.126342
35 Tan H, Yang X, Zhang F, Zheng X, Qu C, Mu J, Fu F, Li J, Guan R, Zhang H, Wang G, Zuo J. Enhanced seed oil production in canola by conditional expression of Brassica napus LEAFY COTYLEDON1 and LEC1-LIKE in developing seeds. Plant Physiology, 2011, 156(3): 1577–1588
https://doi.org/10.1104/pp.111.175000
36 Stone S L, Braybrook S A, Paula S L, Kwong L W, Meuser J, Pelletier J, Hsieh T, Fischer R L, Goldberg R B, Harada J J. Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity: implications for somatic embryogenesis. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(8): 3151–3156
https://doi.org/10.1073/pnas.0712364105
37 Shen B, Allen W B, Zheng P Z, Li C J, Glassman K, Ranch J, Nubel D, Tarczynski M C. Expression of ZmLEC1 and ZmWRI1 increases seed oil production in maize. Plant Physiology, 2010, 153(3): 980–987
https://doi.org/10.1104/pp.110.157537
38 Kim H U, Jung S J, Lee K R, Kim E H, Lee S M, Roh K H, Kim J B. Ectopic overexpression of castor bean LEAFY COTYLEDON2 (LEC2) in Arabidopsis triggers the expression of genes that encode regulators of seed maturation and oil body proteins in vegetative tissues. FEBS Open Bio, 2014, 4(1): 25–32
https://doi.org/10.1016/j.fob.2013.11.003
39 Kagaya Y, Toyoshima R, Okuda R, Usui H, Yamamoto A, Hattori T. LEAFY COTYLEDON1 controls seed storage protein genes through its regulation of FUSCA3 and ABSCISIC ACID INSENSITIVE3. Plant & Cell Physiology, 2005, 46(3): 399–406
https://doi.org/10.1093/pcp/pci048
40 Wang Z, Chen M, Chen T, Xuan L, Li Z, Du X, Zhou L, Zhang G, Jiang L. TRANSPARENT TESTA2 regulates embryonic fatty acid biosynthesis by targeting FUSCA3 during the early developmental stage of Arabidopsis seeds. Plant Journal, 2014, 77(5): 757–769
https://doi.org/10.1111/tpj.12426
49 Jofuku K D, Omidyar P K, Gee Z, Okamuro J K. Control of seed mass and seed yield by the floral homeotic gene APETALA2. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(8): 3117–3122
https://doi.org/10.1073/pnas.0409893102
48 Adamski N M, Anastasiou E, Eriksson S, O’Neill C M, Lenhard M. Local maternal control of seed size by KLUH/CYP78A5-dependent growth signaling. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(47): 20115–20120
https://doi.org/10.1073/pnas.0907024106
53 Chen M X, Xuan L J, Wang Z, Zhou L H, Li Z L, Du X, Ali E, Zhang G P, Jiang L X. TRANSPARENT TESTA 8 inhibits seed fatty acid accumulation by targeting several seed development regulators in Arabidopsis. Plant Physiology, 2014, 165(2): 905–916
https://doi.org/10.1104/pp.114.235507
51 Liu J, Hua W, Yang H L, Zhan G M, Li R J, Deng L B, Wang X F, Liu G H, Wang H Z. The BnGRF2 gene (GRF2-like gene from Brassica napus) enhances seed oil production through regulating cell number and plant photosynthesis. Journal of Experimental Botany, 2012, 63(10): 3727–3740
https://doi.org/10.1093/jxb/ers066
55 Hao W J. Molecular mechanism of cytoplasmic effects on oil content in Brassica Napus. Dissertation for the Doctoral Degree.Wuhan: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 2014 (in Chinese)
41 Roach D A, Wulff R D. Maternal effects in plant. Annual Review of Ecology and Systematics, 1987, 18(1): 209–235
https://doi.org/10.1146/annurev.es.18.110187.001233
42 King S P, Lunn J E, Furbank R T. Carbohydrate content and enzyme metabolism in developing canola siliques. Plant Physiology, 1997, 114(1): 153–160
43 Bennett E J, Roberts J A, Wagstaff C. The role of the pod in seed development: strategies for manipulating yield. New Phytologist, 2011, 190(4): 838–853
https://doi.org/10.1111/j.1469-8137.2011.03714.x
44 Hua W, Li R J, Zhan G M, Liu J, Li J, Wang X F, Liu G H, Wang H Z. Maternal control of seed oil content in Brassica napus: the role of silique wall photosynthesis. Plant Journal, 2012, 69(3): 432–444
https://doi.org/10.1111/j.1365-313X.2011.04802.x
45 Cheng W H, Taliercio E W, Chourey P S. The miniature1 seed locus of maize encodes a cell wall invertase required for normal development of endosperm and maternal cells in the pedicel. Plant Cell, 1996, 8(6): 971–983
https://doi.org/10.1105/tpc.8.6.971
46 Li L, Zhao Y, McCaig B C, Wingerd B A, Wang J, Whalon M E, Pichersky E, Howe G A. The tomato homolog of CORONATINE INSENSITIVE1 is required for the maternal control of seed maturation, jasmonate signaled defense responses, and glandular trichome development. Plant Cell, 2004, 16(1): 126–143
https://doi.org/10.1105/tpc.017954
47 Maitz M, Santandrea G, Zhang Z, Lal S, Hannah L C, Salamini F, Thompson R D. <?Pub Caret?>rgf1, a mutation reducing grain filling in maize through effects on basal endosperm and pedicel development. Plant Journal, 2000, 23(1): 29–42
https://doi.org/10.1046/j.1365-313x.2000.00747.x
50 Ohto M, Floyd S K, Fischer R L, Goldberg R B, Harada J J. Effect of APETALA2 on embryo, endosperm, and seed coat development determine seed size in Arabidopsis. Sexual Plant Reproduction, 2009, 22(4): 277–289
https://doi.org/10.1007/s00497-009-0116-1
52 Liu J, Hua W, Yang H L, Guo T T, Sun X C, Wang X F, Liu G H, Wang H Z. Effects of specific organs on seed oil accumulation in Brassica napus L. Plant Science, 2014, 227(5): 60–68
https://doi.org/10.1016/j.plantsci.2014.06.017
54 Hao W J, Fan S, Hua W, Wang H Z. Effective extraction and assembly methods for simultaneously obtaining plastid and Mitochondrial Genomes. PLoS ONE, 2014, 9(9): e108291
https://doi.org/10.1371/journal.pone.0108291
56 Ecke W, Uzunova M, Wiessleder K. Mapping the genome of rapeseed (Brassica napus L.). II. Localisation of genes controlling erucic acid synthesis and seed oil content. Theoretical and Applied Genetics, 1995, 91(6–7): 972–977
57 Burns M J, Barnes S R, Bowman J G, Clarke M H E, Werner C P, Kearsey M J. QTL analysis of an intervarietal set of substitution lines in Brassica napus: (i) Seed oil content and fatty acid composition. Heredity, 2003, 90(1): 39–48
https://doi.org/10.1038/sj.hdy.6800176
58 Qiu D, Morgan C, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I. A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theoretical and Applied Genetics, 2006, 114(1): 67–80
https://doi.org/10.1007/s00122-006-0411-2
59 Delourme R, Falentin C, Huteau V, Clouet V, Horvais R, Gandon B, Specel S, Hanneton L, Dheu J E, Deschamps M, Margale E, Vincourt P, Renard M. Genetic control of oil content in oilseed rape (Brassica napus L.). Theoretical and Applied Genetics, 2006, 113(7): 1331–1345
https://doi.org/10.1007/s00122-006-0386-z
60 Zhao J Y, Becker H C, Zhang D Q, Zhang Y F, Ecke W. Oil content in a European×Chinese rapeseed population: QTL with additive and epistatic effects and their genotype-environment interactions. Crop Science, 2005, 45(1): 51–59
61 Zhao J Y, Becker H C, Zhang D Q, Zhang Y F, Ecke W. Conditional QTL mapping of oil content in rapeseed with respect to protein content and traits related to plant development and grain yield. Theoretical and Applied Genetics, 2006, 113(1): 33–38
https://doi.org/10.1007/s00122-006-0267-5
62 Zhao J, Huang J, Chen F, Xu F, Ni X Y, Xu H M, Wang Y L, Jiang C C, Wang H, Xu A X, Huang R Z, Li D R, Meng J L. Molecular mapping of Arabidopsis thaliana lipid-related orthologous genes in Brassica napus. Theoretical and Applied Genetics, 2012, 124(2): 407–421
https://doi.org/10.1007/s00122-011-1716-3
63 Jin M Y, Li J N, Fu Y F, Zhang Z S, Zhang X K, Liu L Z. Analysis of the oil content and the hull content in Brassica napus L. Agricultural Sciences in China, 2007, 6(4): 414–421 (in Chinese)
https://doi.org/10.1016/S1671-2927(07)60064-9
64 Zhang J F, Qi C K, Pu H M, Chen S, Chen F, Gao J Q, Chen X J, Gu H, Fu S Z. Inheritance and QTL identification of oil content in rapeseed Brassica napus L. Acta Agronomica Sinica, 2007, 33(9): 1495–1501 (in Chinese)
65 Yan X, Li J, Fu F, Jin M, Chen L, Liu Z. Co-location of seed oil content, seed hull content and seed coat color QTL in three different environments in Brassica napus L. Euphytica, 2009, 170(3): 355–364
https://doi.org/10.1007/s10681-009-0006-5
66 Chen G, Geng J, Rahman M, Liu X, Tu J, Fu T D, Li G Y, McVetty P B E, Tahir M. Identification of QTL for oil content, seed yield, and flowering time in oilseed rape (Brassica napus). Euphytica, 2010, 175(2): 161–174
https://doi.org/10.1007/s10681-010-0144-9
67 Zou J, Jiang C, Cao Z, Li R, Long Y, Chen S, Meng J. Association mapping of seed oil content in different Brassica napus populations and its coincidence with QTL identified from linkage mapping. Genome, 2010, 53: 908–916
68 Sun Z Y, Cheng S, Wang J B, Huang J X, Chen F, Ni X Y, Zhao J Y. Validation of QTL for oil content in a population of worldwide rapeseed cultivars by association analysis. Scientia Agricultura Sinica, 2012, 45(19): 3921–3931 (in Chinese)
69 Sun M Y, Hua W, Liu J, Huang S M, Wang X F, Liu G H, Wang H Z. Design of new genome- and gene-sourced primers and identification of QTL for seed oil content in a specially high-oil Brassica napus cultivar. PLoS ONE, 2012, 7(10): e47037
https://doi.org/10.1371/journal.pone.0047037
70 Sun F, Liu J, Sun X, Wang X, Liu S, Wang H, Hua W. Identification of QTL for seed oil content in high-oil Brassica napus cultivars. Hefei: Plant genomics in China XV, 2014, 99
74 Li F, Chen B Y, Xu K, Wu J F, Song W L, Bancroft I, Harper A L, Trick M, Liu S Y, Gao G Z, Wang N, Yan G X, Qiao J W, Li J, Li H, Xiao X, Zhang T Y, Wu X M. Genome-wide wide association association study study dissects the genetic architecture of seed weight and seed quality in rapeseed (Brassica napus L.). DNA Research, 2014, 21(4): 355–367
https://doi.org/10.1093/dnares/dsu002
71 Li Z F, Xiang J B. Analysis on grey correlative degree of the oil content in rape seed and yield traits. Journal of Mianyang College of Economy & Technology, 1999, 16(1): 16–19 (in Chinese)
72 Sun M Y. Mapping of QTLs and screening of candidate genes for oil content in Brassica napus. Dissertation for the Doctoral Degree.Wuhan: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 2012 (in Chinese)
73 Yang X D. The study on the relationship between lignin biosynthesis manipulation and Brassica napus’ resistance to Sclerotinia sclerotiorum and lodging. Dissertation for the Doctoral Degree. Wuhan: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 2006 (in Chinese)
75 Rahman H, Harwood J, Weselake R. Increasing seed oil content in Brassica species through breeding and biotechnology. Lipid Technology, 2013, 25(8): 182–185
https://doi.org/10.1002/lite.201300291
76 Baud S, Lepiniec L. Physiological and developmental regulation of seed oil production. Progress in Lipid Research, 2010, 49(3): 235–249
https://doi.org/10.1016/j.plipres.2010.01.001
77 Vuorinen A L, Kalpio M, Linderborg K M, Kortesniemi M, Lehto K, Niemi J, Yang B, Kallio H P. Coordinate changes in gene expression and triacylglycerol composition in the developing seeds of oilseed rape (Brassica napus) and turnip rape (Brassica rapa). Food Chemistry, 2014, 145(4): 664–673
https://doi.org/10.1016/j.foodchem.2013.08.108
78 Yu E, Fan C C, Yang Q Y, Li X D, Wan B X, Dong Y N, Wang X M, Zhou Y M. Identification of heat responsive genes in Brassica napus siliques at the seed-filling stage through transcriptional profiling. PLoS ONE, 2014, 9(7): e101914
https://doi.org/10.1371/journal.pone.0101914
79 Fu T D. Improvement of rapeseed varities. Crop Research, 2007, (3): 159–162
80 Fu S H, Zhang J F, Qi C K, Pu H M, Gao Q J, Chen X J, Chen F. Breeding for high oil content lines in rapeseed (Brassica napus L.). Chinese Journal of Oil Crop Sciences, 2008, 30(3): 279–283 (in Chinese)
81 Zhu J C, Zhang S F, Wen Y C, Wang J P, Zhao L, Wang J L, Liu G. Studies on breeding method of the high oil content line T057–7 in Brassica napus. Chinese Agricultural Science Bulletin, 2009, 25(18): 194–197 (in Chinese)
82 Li D R, Tian J H, Chen W J, Zhang W X, Li Y H, Wang H. Breeding technologies and germplasm innovation on extra-high-oil content in Brassica napus. Acta Agriculturae Boreali-occidentalis Sinica, 2011, 20(12): 83–87 (in Chinese)
83 McVetty P B E, Scarth R, Fernando W G D, Li G, Sun Z, Taylor D, Tu J, Zelmer C D. Brassica seed quality breeding at the University of Manitoba. In: The 12th international Rapeseed Congress, Wuhan. The Groupe Consultatif International de Recherche sur le Colza (GCIRC) (International Consultative Group of Research on Rapeseed), 2007, 2–4
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