This picture describes the general procedures for producing genome modified animals with CRISPR/Cas9 system. In the system, Cas9 and sgRNA are used to make site-specific double strand breaks and the targeting donor template is used to bring specific mutations via homology-directed repair mechanism. The genome modified animals can either be produced by direct injection of plasmids into zygotes followed by embryo transfer or transfection and selection of somatic cells followed by somatic cell cloning
(Shaohua WANG, Kun ZHANG, Yunping DAI, pp. 1–10)