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2014年, 第1卷 第1期
Gene targeting in large domestic animals has been considered an intractable task involving screening for gene-targeted cells and somatic cell nuclear transfer (SCNT), and it frequently results in abortion or unhealthy newborns due to abnormal epigenetic modifications. Here, we report the first successful generation of gene-knockout sheep using a one-step zygote injection of the CRISPR/Cas9 system. (Hongbing HAN, Yonghe MA, Tao WANG, Ling LIAN, Xiuzhi TIAN, Rui HU, Shoulong DENG, Kongpan LI, Feng WANG, Ning LI, Guoshi LIU, Yaofeng ZHAO, Zhengxing LIAN, pp. 2-5)[展开] ...