A brief review of genome editing technology for generating animal models

doi:10.15302/J-FASE-2019309

1	R Jaenisch , B Mintz . Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proceedings of the National Academy of Sciences of the United States of America, 1974, 71(4): 1250–1254
2	R Jaenisch. Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus. Proceedings of the National Academy of Sciences of the United States of America, 1976, 73(4): 1260–1264 https://doi.org/10.1073/pnas.73.4.1260 pmid: 1063407
3	J W Gordon, G A Scangos, D J Plotkin, J A Barbosa, F H Ruddle. Genetic transformation of mouse embryos by microinjection of purified DNA. Proceedings of the National Academy of Sciences of the United States of America, 1980, 77(12): 7380–7384 https://doi.org/10.1073/pnas.77.12.7380 pmid: 6261253
4	A Schnieke, K Harbers, R Jaenisch. Embryonic lethal mutation in mice induced by retrovirus insertion into the α1(I) collagen gene. Nature, 1983, 304(5924): 315–320 https://doi.org/10.1038/304315a0 pmid: 6308457
5	O Smithies, R G Gregg, S S Boggs, M A Koralewski, R S Kucherlapati. Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombination. Nature, 1985, 317(6034): 230–234 https://doi.org/10.1038/317230a0 pmid: 2995814
6	K R Thomas, K R Folger, M R Capecchi. High frequency targeting of genes to specific sites in the mammalian genome. Cell, 1986, 44(3): 419–428 https://doi.org/10.1016/0092-8674(86)90463-0 pmid: 3002636
7	M R Capecchi. Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nature Reviews: Genetics, 2005, 6(6): 507–512 https://doi.org/10.1038/nrg1619 pmid: 15931173
8	A Bradley, M Evans, M H Kaufman, E Robertson. Formation of germ-line chimaeras from embryo-derived teratocarcinoma cell lines. Nature, 1984, 309(5965): 255–256 https://doi.org/10.1038/309255a0 pmid: 6717601
9	K R Thomas, M R Capecchi. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell, 1987, 51(3): 503–512 https://doi.org/10.1016/0092-8674(87)90646-5 pmid: 2822260
10	T Doetschman, R G Gregg, N Maeda, M L Hooper, D W Melton, S Thompson, O Smithies. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature, 1987, 330(6148): 576–578 https://doi.org/10.1038/330576a0 pmid: 3683574
11	M Buehr, S Meek, K Blair, J Yang, J Ure, J Silva, R McLay, J Hall, Q L Ying, A Smith. Capture of authentic embryonic stem cells from rat blastocysts. Cell, 2008, 135(7): 1287–1298 https://doi.org/10.1016/j.cell.2008.12.007 pmid: 19109897
12	P Rouet, F Smih, M Jasin. Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease. Molecular and Cellular Biology, 1994, 14(12): 8096–8106 https://doi.org/10.1128/MCB.14.12.8096 pmid: 7969147
13	N Rudin, E Sugarman, J E Haber. Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae. Genetics, 1989, 122(3): 519–534 pmid: 2668114
14	G Silva, L Poirot, R Galetto, J Smith, G Montoya, P Duchateau, F Pâques. Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy. Current Gene Therapy, 2011, 11(1): 11–27 https://doi.org/10.2174/156652311794520111 pmid: 21182466
15	J Miller, A D McLachlan, A Klug. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO Journal, 1985, 4(6): 1609–1614 https://doi.org/10.1002/j.1460-2075.1985.tb03825.x pmid: 4040853
16	H S Najafabadi, S Mnaimneh, F W Schmitges, M Garton, K N Lam, A Yang, M Albu, M T Weirauch, E Radovani, P M Kim, J Greenblatt, B J Frey, T R Hughes. C2H2 zinc finger proteins greatly expand the human regulatory lexicon. Nature Biotechnology, 2015, 33(5): 555–562 https://doi.org/10.1038/nbt.3128 pmid: 25690854
17	H Takatsuji. Zinc-finger transcription factors in plants. Cellular and Molecular Life Sciences, 1998, 54(6): 582–596 https://doi.org/10.1007/s000180050186 pmid: 9676577
18	F D Urnov, E J Rebar, M C Holmes, H S Zhang, P D Gregory. Genome editing with engineered zinc finger nucleases. Nature Reviews: Genetics, 2010, 11(9): 636–646 https://doi.org/10.1038/nrg2842 pmid: 20717154
19	M Bibikova, D Carroll, D J Segal, J K Trautman, J Smith, Y G Kim, S Chandrasegaran. Stimulation of homologous recombination through targeted cleavage by chimeric nucleases. Molecular and Cellular Biology, 2001, 21(1): 289–297 https://doi.org/10.1128/MCB.21.1.289-297.2001 pmid: 11113203
20	M Bibikova, M Golic, K G Golic, D Carroll. Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics, 2002, 161(3): 1169–1175 pmid: 12136019
21	M Bibikova, K Beumer, J K Trautman, D Carroll. Enhancing gene targeting with designed zinc finger nucleases. Science, 2003, 300(5620): 764 https://doi.org/10.1126/science.1079512 pmid: 12730594
22	K J Beumer, J K Trautman, A Bozas, J L Liu, J Rutter, J G Gall, D Carroll. Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(50): 19821–19826 https://doi.org/10.1073/pnas.0810475105 pmid: 19064913
23	Y Doyon, J M McCammon, J C Miller, F Faraji, C Ngo, G E Katibah, R Amora, T D Hocking, L Zhang, E J Rebar, P D Gregory, F D Urnov, S L Amacher. Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases. Nature Biotechnology, 2008, 26(6): 702–708 https://doi.org/10.1038/nbt1409 pmid: 18500334
24	X Meng, M B Noyes, L J Zhu, N D Lawson, S A Wolfe. Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nature Biotechnology, 2008, 26(6): 695–701 https://doi.org/10.1038/nbt1398 pmid: 18500337
25	A M Geurts, G J Cost, Y Freyvert, B Zeitler, J C Miller, V M Choi, S S Jenkins, A Wood, X Cui, X Meng, A Vincent, S Lam, M Michalkiewicz, R Schilling, J Foeckler, S Kalloway, H Weiler, S Ménoret, I Anegon, G D Davis, L Zhang, E J Rebar, P D Gregory, F D Urnov, H J Jacob, R Buelow. Knockout rats via embryo microinjection of zinc-finger nucleases. Science, 2009, 325(5939): 433 https://doi.org/10.1126/science.1172447 pmid: 19628861
26	J Boch, H Scholze, S Schornack, A Landgraf, S Hahn, S Kay, T Lahaye, A Nickstadt, U Bonas. Breaking the code of DNA binding specificity of TAL-type III effectors. Science, 2009, 326(5959): 1509–1512 https://doi.org/10.1126/science.1178811 pmid: 19933107
27	M J Moscou, A J Bogdanove. A simple cipher governs DNA recognition by TAL effectors. Science, 2009, 326(5959): 1501 https://doi.org/10.1126/science.1178817 pmid: 19933106
28	J C Miller, S Tan, G Qiao, K A Barlow, J Wang, D F Xia, X Meng, D E Paschon, E Leung, S J Hinkley, G P Dulay, K L Hua, I Ankoudinova, G J Cost, F D Urnov, H S Zhang, M C Holmes, L Zhang, P D Gregory, E J Rebar. A TALE nuclease architecture for efficient genome editing. Nature Biotechnology, 2011, 29(2): 143–148 https://doi.org/10.1038/nbt.1755 pmid: 21179091
29	D Hockemeyer, H Wang, S Kiani, C S Lai, Q Gao, J P Cassady, G J Cost, L Zhang, Y Santiago, J C Miller, B Zeitler, J M Cherone, X Meng, S J Hinkley, E J Rebar, P D Gregory, F D Urnov, R Jaenisch. Genetic engineering of human pluripotent cells using TALE nucleases. Nature Biotechnology, 2011, 29(8): 731–734 https://doi.org/10.1038/nbt.1927 pmid: 21738127
30	L Tesson, C Usal, S Ménoret, E Leung, B J Niles, S Remy, Y Santiago, A I Vincent, X Meng, L Zhang, P D Gregory, I Anegon, G J Cost. Knockout rats generated by embryo microinjection of TALENs. Nature Biotechnology, 2011, 29(8): 695–696 https://doi.org/10.1038/nbt.1940 pmid: 21822240
31	J D Sander, L Cade, C Khayter, D Reyon, R T Peterson, J K Joung, J R J Yeh. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology, 2011, 29(8): 697–698 https://doi.org/10.1038/nbt.1934 pmid: 21822241
32	P Huang, A Xiao, M Zhou, Z Zhu, S Lin, B Zhang. Heritable gene targeting in zebrafish using customized TALENs. Nature Biotechnology, 2011, 29(8): 699–700 https://doi.org/10.1038/nbt.1939 pmid: 21822242
33	Z Qiu, M Liu, Z Chen, Y Shao, H Pan, G Wei, C Yu, L Zhang, X Li, P Wang, H Y Fan, B Du, B Liu, M Liu, D Li. High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases. Nucleic Acids Research, 2013, 41(11): e120 https://doi.org/10.1093/nar/gkt258 pmid: 23630316
34	H Liu, Y Chen, Y Niu, K Zhang, Y Kang, W Ge, X Liu, E Zhao, C Wang, S Lin, B Jing, C Si, Q Lin, X Chen, H Lin, X Pu, Y Wang, B Qin, F Wang, H Wang, W Si, J Zhou, T Tan, T Li, S Ji, Z Xue, Y Luo, L Cheng, Q Zhou, S Li, Y E Sun, W Ji. TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys. Cell Stem Cell, 2014, 14(3): 323–328 https://doi.org/10.1016/j.stem.2014.01.018 pmid: 24529597
35	S Remy, L Tesson, S Menoret, C Usal, A De Cian, V Thepenier, R Thinard, D Baron, M Charpentier, J B Renaud, R Buelow, G J Cost, C Giovannangeli, A Fraichard, J P Concordet, I Anegon. Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases. Genome Research, 2014, 24(8): 1371–1383 https://doi.org/10.1101/gr.171538.113 pmid: 24989021
36	B Wefers, M Meyer, O Ortiz, M Hrabé de Angelis, J Hansen, W Wurst, R Kühn. Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(10): 3782–3787 https://doi.org/10.1073/pnas.1218721110 pmid: 23426636
37	V M Bedell, Y Wang, J M Campbell, T L Poshusta, C G Starker, R G 2nd Krug, W Tan, S G Penheiter, A C Ma, A Y H Leung, S C Fahrenkrug, D F Carlson, D F Voytas, K J Clark, J J Essner, S C Ekker. In vivo genome editing using a high-efficiency TALEN system. Nature, 2012, 491(7422): 114–118 https://doi.org/10.1038/nature11537 pmid: 23000899
38	H Wang, Y C Hu, S Markoulaki, G G Welstead, A W Cheng, C S Shivalila, T Pyntikova, D B Dadon, D F Voytas, A J Bogdanove, D C Page, R Jaenisch. TALEN-mediated editing of the mouse Y chromosome. Nature Biotechnology, 2013, 31(6): 530–532 https://doi.org/10.1038/nbt.2595 pmid: 23666012
39	W Tan, D F Carlson, C A Lancto, J R Garbe, D A Webster, P B Hackett, S C Fahrenkrug. Efficient nonmeiotic allele introgression in livestock using custom endonucleases. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(41): 16526–16531 https://doi.org/10.1073/pnas.1310478110 pmid: 24014591
40	D F Carlson, W Tan, S G Lillico, D Stverakova, C Proudfoot, M Christian, D F Voytas, C R Long, C B Whitelaw, S C Fahrenkrug. Efficient TALEN-mediated gene knockout in livestock. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(43): 17382–17387 https://doi.org/10.1073/pnas.1211446109 pmid: 23027955
41	K S Makarova, Y I Wolf, O S Alkhnbashi, F Costa, S A Shah, S J Saunders, R Barrangou, S J Brouns, E Charpentier, D H Haft, P Horvath, S Moineau, F J Mojica, R M Terns, M P Terns, M F White, A F Yakunin, R A Garrett, J van der Oost, R Backofen, E V Koonin. An updated evolutionary classification of CRISPR-Cas systems. Nature Reviews: Microbiology, 2015, 13(11): 722–736 https://doi.org/10.1038/nrmicro3569 pmid: 26411297
42	F Jiang, J A Doudna. CRISPR-Cas9 structures and mechanisms. Annual Review of Biophysics, 2017, 46(1): 505–529 https://doi.org/10.1146/annurev-biophys-062215-010822 pmid: 28375731
43	G Gasiunas, R Barrangou, P Horvath, V Siksnys. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(39): E2579–E2586 https://doi.org/10.1073/pnas.1208507109 pmid: 22949671
44	M Jinek, K Chylinski, I Fonfara, M Hauer, J A Doudna, E Charpentier. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 2012, 337(6096): 816–821 https://doi.org/10.1126/science.1225829 pmid: 22745249
45	P Mali, L Yang, K M Esvelt, J Aach, M Guell, J E DiCarlo, J E Norville, G M Church. RNA-guided human genome engineering via Cas9. Science, 2013, 339(6121): 823–826 https://doi.org/10.1126/science.1232033 pmid: 23287722
46	L Cong, F A Ran, D Cox, S Lin, R Barretto, N Habib, P D Hsu, X Wu, W Jiang, L A Marraffini, F Zhang. Multiplex genome engineering using CRISPR/Cas systems. Science, 2013, 339(6121): 819–823 https://doi.org/10.1126/science.1231143 pmid: 23287718
47	H Wang, H Yang, C S Shivalila, M M Dawlaty, A W Cheng, F Zhang, R Jaenisch. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell, 2013, 153(4): 910–918 https://doi.org/10.1016/j.cell.2013.04.025 pmid: 23643243
48	L Yang, M Güell, D Niu, H George, E Lesha, D Grishin, J Aach, E Shrock, W Xu, J Poci, R Cortazio, R A Wilkinson, J A Fishman, G Church. Genome-wide inactivation of porcine endogenous retroviruses (PERVs). Science, 2015, 350(6264): 1101–1104 https://doi.org/10.1126/science.aad1191 pmid: 26456528
49	H Yang, H Wang, C S Shivalila, A W Cheng, L Shi, R Jaenisch. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell, 2013, 154(6): 1370–1379 https://doi.org/10.1016/j.cell.2013.08.022 pmid: 23992847
50	W Li, F Teng, T Li, Q Zhou. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nature Biotechnology, 2013, 31(8): 684–686 https://doi.org/10.1038/nbt.2652 pmid: 23929337
51	D Li, Z Qiu, Y Shao, Y Chen, Y Guan, M Liu, Y Li, N Gao, L Wang, X Lu, Y Zhao, M Liu. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nature Biotechnology, 2013, 31(8): 681–683 https://doi.org/10.1038/nbt.2661 pmid: 23929336
52	W Y Hwang, Y Fu, D Reyon, M L Maeder, S Q Tsai, J D Sander, R T Peterson, J R Yeh, J K Joung. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nature Biotechnology, 2013, 31(3): 227–229 https://doi.org/10.1038/nbt.2501 pmid: 23360964
53	G Xiang, J Ren, T Hai, R Fu, D Yu, J Wang, W Li, H Wang, Q Zhou. Editing porcine IGF2 regulatory element improved meat production in Chinese Bama pigs. Cellular and Molecular Life Sciences, 2018, 75(24): 4619–4628 https://doi.org/10.1007/s00018-018-2917-6 pmid: 30259067
54	Y Niu, B Shen, Y Cui, Y Chen, J Wang, L Wang, Y Kang, X Zhao, W Si, W Li, A P Xiang, J Zhou, X Guo, Y Bi, C Si, B Hu, G Dong, H Wang, Z Zhou, T Li, T Tan, X Pu, F Wang, S Ji, Q Zhou, X Huang, W Ji, J Sha. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell, 2014, 156(4): 836–843 https://doi.org/10.1016/j.cell.2014.01.027 pmid: 24486104
55	M Hashimoto, T Takemoto. Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific Reports, 2015, 5(1): 11315 https://doi.org/10.1038/srep11315 pmid: 26066060
56	W Qin, S L Dion, P M Kutny, Y Zhang, A W Cheng, N L Jillette, A Malhotra, A M Geurts, Y G Chen, H Wang. Efficient CRISPR/Cas9-mediated genome editing in mice by zygote electroporation of nuclease. Genetics, 2015, 200(2): 423–430 https://doi.org/10.1534/genetics.115.176594 pmid: 25819794
57	T Kaneko, T Sakuma, T Yamamoto, T Mashimo. Simple knockout by electroporation of engineered endonucleases into intact rat embryos. Scientific Reports, 2014, 4(1): 6382 https://doi.org/10.1038/srep06382 pmid: 25269785
58	W Wang, P M Kutny, S L Byers, C J Longstaff, M J DaCosta, C Pang, Y Zhang, R A Taft, F W Buaas, H Wang. Delivery of Cas9 protein into mouse zygotes through a series of electroporation dramatically increases the efficiency of model creation. Journal of Genetics and Genomics, 2016, 43(5): 319–327 https://doi.org/10.1016/j.jgg.2016.02.004 pmid: 27210041
59	G Takahashi, C B Gurumurthy, K Wada, H Miura, M Sato, M Ohtsuka. GONAD: genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice. Scientific Reports, 2015, 5(1): 11406 https://doi.org/10.1038/srep11406 pmid: 26096991
60	Y Wu, H Zhou, X Fan, Y Zhang, M Zhang, Y Wang, Z Xie, M Bai, Q Yin, D Liang, W Tang, J Liao, C Zhou, W Liu, P Zhu, H Guo, H Pan, C Wu, H Shi, L Wu, F Tang, J Li. Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells. Cell Research, 2015, 25(1): 67–79 https://doi.org/10.1038/cr.2014.160 pmid: 25475058
61	L Wei, X Wang, S Yang, W Yuan, J Li. Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene editing. Journal of Genetics and Genomics, 2017, 44(9): 461–463 https://doi.org/10.1016/j.jgg.2017.07.004 pmid: 28943147
62	M Adli. The CRISPR tool kit for genome editing and beyond. Nature Communications, 2018, 9(1): 1911 https://doi.org/10.1038/s41467-018-04252-2 pmid: 29765029
63	H Wang, M La Russa, L S Qi. CRISPR/Cas9 in genome editing and beyond. Annual Review of Biochemistry, 2016, 85(1): 227–264 https://doi.org/10.1146/annurev-biochem-060815-014607 pmid: 27145843
64	H A Rees, D R Liu. Base editing: precision chemistry on the genome and transcriptome of living cells. Nature Reviews: Genetics, 2018, 19(12): 770–788 https://doi.org/10.1038/s41576-018-0059-1 pmid: 30323312
65	K Nishida, T Arazoe, N Yachie, S Banno, M Kakimoto, M Tabata, M Mochizuki, A Miyabe, M Araki, K Y Hara, Z Shimatani, A Kondo. Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science, 2016, 353(6305): aaf8729 https://doi.org/10.1126/science.aaf8729 pmid: 27492474
66	A C Komor, Y B Kim, M S Packer, J A Zuris, D R Liu. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Nature, 2016, 533(7603): 420–424 https://doi.org/10.1038/nature17946 pmid: 27096365
67	N M Gaudelli, A C Komor, H A Rees, M S Packer, A H Badran, D I Bryson, D R Liu. Programmable base editing of A·T to G·C in genomic DNA without DNA cleavage. Nature, 2017, 551(7681): 464–471 https://doi.org/10.1038/nature24644 pmid: 29160308
68	Z Liu, Z Lu, G Yang, S Huang, G Li, S Feng, Y Liu, J Li, W Yu, Y Zhang, J Chen, Q Sun, X Huang. Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Nature Communications, 2018, 9(1): 2338 https://doi.org/10.1038/s41467-018-04768-7 pmid: 29904106
69	P Liang, H Sun, Y Sun, X Zhang, X Xie, J Zhang, Z Zhang, Y Chen, C Ding, Y Xiong, W Ma, D Liu, J Huang, Z Songyang. Effective gene editing by high-fidelity base editor 2 in mouse zygotes. Protein & Cell, 2017, 8(8): 601–611 https://doi.org/10.1007/s13238-017-0418-2 pmid: 28585179
70	K Kim, S M Ryu, S T Kim, G Baek, D Kim, K Lim, E Chung, S Kim, J S Kim. Highly efficient RNA-guided base editing in mouse embryos. Nature Biotechnology, 2017, 35(5): 435–437 https://doi.org/10.1038/nbt.3816 pmid: 28244995
71	Y Ma, L Yu, X Zhang, C Xin, S Huang, L Bai, W Chen, R Gao, J Li, S Pan, X Qi, X Huang, L Zhang. Highly efficient and precise base editing by engineered dCas9-guide tRNA adenosine deaminase in rats. Cell Discovery, 2018, 4(1): 39 https://doi.org/10.1038/s41421-018-0047-9 pmid: 30038797
72	Z Liu, M Chen, S Chen, J Deng, Y Song, L Lai, Z Li. Highly efficient RNA-guided base editing in rabbit. Nature Communications, 2018, 9(1): 2717 https://doi.org/10.1038/s41467-018-05232-2 pmid: 30006570
73	L Yang, X Zhang, L Wang, S Yin, B Zhu, L Xie, Q Duan, H Hu, R Zheng, Y Wei, L Peng, H Han, J Zhang, W Qiu, H Geng, S Siwko, X Zhang, M Liu, D Li. Increasing targeting scope of adenosine base editors in mouse and rat embryos through fusion of TadA deaminase with Cas9 variants. Protein & Cell, 2018, 9(9): 814–819 https://doi.org/10.1007/s13238-018-0568-x pmid: 30066232
74	A V Anzalone, P B Randolph, J R Davis, A A Sousa, L W Koblan, J M Levy, P J Chen, C Wilson, G A Newby, A Raguram, D R Liu. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 2019, 576(7785): 149–157 https://doi.org/10.1038/s41586-019-1711-4 pmid: 31634902
75	S E Klompe, P L H Vo, T S Halpin-Healy, S H Sternberg. Transposon-encoded CRISPR-Cas systems direct RNA-guided DNA integration. Nature, 2019, 571(7764): 219–225 https://doi.org/10.1038/s41586-019-1323-z pmid: 31189177
76	J Strecker, A Ladha, Z Gardner, J L Schmid-Burgk, K S Makarova, E V Koonin, F Zhang. RNA-guided DNA insertion with CRISPR-associated transposases. Science, 2019, 365(6448): 48–53 https://doi.org/10.1126/science.aax9181 pmid: 31171706