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Frontiers of Agricultural Science and Engineering    2019, Vol. 6 Issue (3) : 225-232     https://doi.org/10.15302/J-FASE-2019265
REVIEW
Wheat research and breeding in the new era of a high-quality reference genome
Rudi APPELS1,2()
1. Centre for AgriBioscience/Department of Economic Development, LaTrobe University, Bundoora VIC 3083, Australia
2. Department of BioSciences, The University of Melbourne, Victoria 2052, Australia
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Abstract

The publications of the International Wheat Genome Sequencing Consortium (IWGSC) released in August 2018 are reviewed and placed into the context of developments arising from the availability of the high-quality wheat genome assembly.

Keywords assembly technology      molecular markers      polyploidy      transcript networks      wheat genome     
最新录用日期:    在线预览日期:    发布日期: 2019-07-26
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Rudi APPELS
引用本文:   
Rudi APPELS. Wheat research and breeding in the new era of a high-quality reference genome[J]. Front. Agr. Sci. Eng. , 2019, 6(3): 225-232.
网址:  
https://journal.hep.com.cn/fase/EN/10.15302/J-FASE-2019265     OR     https://journal.hep.com.cn/fase/EN/Y2019/V6/I3/225
Fig.1  A representative view of c. 200 kb of the wheat genome showing the gene islands (blue) and retrotransposable elements (pink). The scale near the top of the image is in the genome position scale base pairs. The image is a screen capture of IWGSC RefSeq v1.0 viewed in Apollo[19] as established in the Earlham Institute, Norwich Research Park, UK.
Fig.2  Detail of a 2.4-Mb region from chromosome 7AS (modified from Keeble-Gagnère et al.[4]). The matching regions from IWGSC RefSeq v1.0 (orange), TGAC (cyan) and PacBio (yellow) assemblies show the alignments of the genome sequences and the black bars indicate differences between genome sequences. The vertical pink bars indicate regions of the finished sequence not present in any other assembly.
Fig.3  Summary of overall diversity in gene numbers within the wheat genome: modified from IWGSC[1]. The ratio, 1:1:1, indicates homeologous genes present on the A, B and D genomes. The ratio, 1:1:N, indicates the respective homeologous gene on the D genome has multiple copies, similarly for 1:N:1 and N:1:1 designators as indicated in the table. The ratio, 1:1:0, indicates the respective homeologous gene on the D genome is absent, similarly for 1:0:1 and 0:1:1 designators as indicated in the table.
Fig.4  Synteny alignment of a well-known rice blast locus on rice chromosome 8 to a new location on the long arm of wheat chromosome 5A. The synteny based alignment was performed with Pretzel software[33].
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